Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus

被引：62

作者：

Jonas, D

Grundmann, H

Hartung, D

Daschner, FD

Towner, KJ

机构：

[1] Univ Hosp Freiburg, Inst Environm Med & Hosp Epidemiol, D-79106 Freiburg, Germany

[2] Univ Nottingham Hosp, Queens Med Ctr, Dept Microbiol, Nottingham NG7 2UH, England

[3] Univ Nottingham Hosp, Queens Med Ctr, Publ Hlth Lab Serv Lab, Nottingham NG7 2UH, England

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1999年 / 18卷 / 09期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1007/s100960050365

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This study systematically evaluated a recently described duplex polymerase chain reaction test for methicillin-resistant Staphylococcus aureus with 25 different German epidemic strains of methicillin-resistant Staphylococcus aureus and 66 staphylococci other than methicillin-resistant Staphylococcus aureus, including 17 different coagulase-negative staphylococcal species and subspecies, that were either oxacillin susceptible or oxacillin resistant, The results were compared with those of conventional cultural identification and susceptibility testing. Of the 91 isolates tested, all 25 confirmed strains of methicillin-resistant Staphylococcus aureus were identified correctly, None of the remaining strains of methicillin-susceptible Staphylococcus aureus was misidentified as methicillin-resistant Staphylococcus aureus. It was concluded that the duplex polymerase chain reaction appears to offer a timesaving and accurate method of detection of methicillin-resistant Staphylococcus. aureus.

引用

页码：643 / 647

页数：5

共 31 条

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