Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat

被引：86

作者：

Liu, Z ^{[1
]}

Sun, Q ^{[1
]}

Ni, Z ^{[1
]}

Yang, T ^{[1
]}

机构：

[1] China Agr Univ, Dept Genet & Plant Breeding, Beijing 100094, Peoples R China

来源：

PLANT BREEDING | 1999年 / 118卷 / 03期

关键词：

Erysiphe (Blumeria) graminis; Haynaldia villosa; Triticum aestivum; marker-assisted selection; Pm21; gene; powdery mildew resistance; RAPD; SCAR;

D O I：

10.1046/j.1439-0523.1999.118003215.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Powdery mildew is an important disease in most of the wheat production areas of the world. The resistance gene Pm21 (6AL/6VS translocation) derived from Haynaldia villosa confers resistance to all available isolates of Erysiphe (Blumeria) graminis f. sp. tritici in China and Europe. The objective of this study was to develop fast and reliable sequence characterized amplified region (SCAR) markers linked to the Pm21 gene. A random amplified polymorphic DNA (RAPD) marker for Pm21, OPH17(1400), was converted to SCAR markers after sequencing the two ends of the polymorphic DNA fragment. Two SCAR markers, SCAR(1265) and SCAR(1400), were developed to detect the Pm21 gene in different genetic backgrounds. The specific SCAR(1265) marker enable large-scale accurate screening for the presence/absence of Pm21 allele.

引用

页码：215 / 219

页数：5

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