Musculin isoforms and repression of MyoD in muscle regeneration

被引:17
作者
Zhao, P [1 ]
Hoffman, EP [1 ]
机构
[1] Childrens Natl Med Ctr, Res Ctr Genet Med, Washington, DC 20010 USA
关键词
muscle regeneration; musculin; MyoR; MyoD; bHLH; isoform;
D O I
10.1016/j.bbrc.2006.01.188
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We studied possible negative regulators of MyoD using a 27 time point muscle regeneration series in vivo and identified Musculin as a key candidate for transcriptional repression of MyoD. Characterization of Musculin proteins showed two isoforms: the previously characterized 201 aa protein (1a) and a novel 180 aa isoform (1b). We show that the Musculin 1b isoform is equally effective at blocking MyoD-induced transcription as the 1a isoform. Our data narrow the likely repressor domain to between position 158 and 173 in the Musculin protein sequence, and suggest that the induction of Musculin is responsible for the rapid down-regulation of MyoD at 4-5 days during staged regeneration. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:835 / 842
页数:8
相关论文
共 24 条
[1]   THE PROTEIN ID - A NEGATIVE REGULATOR OF HELIX-LOOP-HELIX DNA-BINDING PROTEINS [J].
BENEZRA, R ;
DAVIS, RL ;
LOCKSHON, D ;
TURNER, DL ;
WEINTRAUB, H .
CELL, 1990, 61 (01) :49-59
[2]   A network-based analysis of systemic inflammation in humans [J].
Calvano, SE ;
Xiao, WZ ;
Richards, DR ;
Felciano, RM ;
Baker, HV ;
Cho, RJ ;
Chen, RO ;
Brownstein, BH ;
Cobb, JP ;
Tschoeke, SK ;
Miller-Graziano, C ;
Moldawer, LL ;
Mindrinos, MN ;
Davis, RW ;
Tompkins, RG ;
Lowry, SF .
NATURE, 2005, 437 (7061) :1032-1037
[3]   The PEPR GeneChip data warehouse, and implementation of a dynamic time series query tool (SGQT) with graphical interface [J].
Chen, J ;
Zhao, P ;
Massaro, D ;
Clerch, LB ;
Almon, RR ;
DuBois, DC ;
Jusko, WJ ;
Hoffman, EP .
NUCLEIC ACIDS RESEARCH, 2004, 32 :D578-D581
[4]  
Doucet Christine, 2005, BMC Biochem, V6, P27, DOI 10.1186/1471-2091-6-27
[5]   The widespread impact of mammalian microRNAs on mRNA repression and evolution [J].
Farh, KKH ;
Grimson, A ;
Jan, C ;
Lewis, BP ;
Johnston, WK ;
Lim, LP ;
Burge, CB ;
Bartel, DP .
SCIENCE, 2005, 310 (5755) :1817-1821
[6]   HES6 acts as a transcriptional repressor in myoblasts and can induce the myogenic differentiation program [J].
Gao, XM ;
Chandra, T ;
Gratton, MO ;
Quélo, I ;
Prud'homme, J ;
Stifani, S ;
St-Arnaud, R .
JOURNAL OF CELL BIOLOGY, 2001, 154 (06) :1161-1171
[7]   The basic domain of myogenic basic helix-loop-helix (bHLH) proteins is the novel target for direct inhibition by another bHLH protein, Twist [J].
Hamamori, Y ;
Wu, HY ;
Sartorelli, V ;
Kedes, L .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (11) :6563-6573
[8]   Musculin/MyoR is expressed in kidney side population cells and can regulate their function [J].
Hishikawa, K ;
Marumo, T ;
Miura, S ;
Nakanishi, A ;
Matsuzaki, Y ;
Shibata, K ;
Ichiyanagi, T ;
Kohike, H ;
Komori, T ;
Takahashi, I ;
Takase, O ;
Imai, N ;
Yoshikawa, M ;
Inowa, T ;
Hayashi, M ;
Nakaki, T ;
Nakauchi, H ;
Okano, H ;
Fujita, T .
JOURNAL OF CELL BIOLOGY, 2005, 169 (06) :921-928
[9]   OVEREXPRESSION OF ID PROTEIN INHIBITS THE MUSCLE DIFFERENTIATION PROGRAM - INVIVO ASSOCIATION OF ID WITH E2A PROTEINS [J].
JEN, Y ;
WEINTRAUB, H ;
BENEZRA, R .
GENES & DEVELOPMENT, 1992, 6 (08) :1466-1479
[10]   Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice [J].
Kassar-Duchossoy, L ;
Gayraud-Morel, B ;
Gomès, D ;
Rocancourt, D ;
Buckingham, M ;
Shinin, V ;
Tajbakhsh, S .
NATURE, 2004, 431 (7007) :466-471