Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene

被引：27

作者：

Huang, Wei ^{[1
,2
]}

Tian, Xiang-Long ^{[2
]}

Wu, Yun-Lin ^{[2
]}

Zhong, Jie ^{[2
]}

Yu, Li-Fen ^{[2
]}

Hu, Sheng-Ping ^{[1
]}

Li, Biao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Nucl Med, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Gastroenterol, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2008年 / 14卷 / 38期

关键词：

Gastric cancer; Normal epithelial cell specific-1 gene; Baculovirus; Gene therapy;

D O I：

10.3748/wjg.14.5810

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo. METHODS: We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus for gene transfer into SGC-7901 cells and cell growth curves were detected by fluorescence microscopy, Western blot and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro, respectively. The therapeutic effect of this gene therapy on GC was confirmed in xenografted nude mice. Tumor growth was determined by tumor volume, and expression of NES1 in tumor was analyzed by immunohistochemistry. RESULTS: Baculovirus vectors were successfully transfected into SGC-7901 cells. SGC-7901 cells transfected with the NE51 gene inhibited cell growth. In the Bac-NES1 treated group, tumor growth was significantly reduced with a high level of NES1 expression CONCLUSION: Baculovirus-mediated NE51 gene can be used in gene therapy for GC. (C) 2008 The WJG Press. All rights reserved.

引用

页码：5810 / 5815

页数：6

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