Expression of metalloproteinases and their inhibitors in blood vessels in human endometrium

被引:72
作者
Freitas, S
Meduri, G
Le Nestour, E
Bausero, P
Perrot-Applanat, M [1 ]
机构
[1] CHU Xavier Bichat, INSERM, U460, F-75870 Paris, France
[2] Lab Cassenne Hoechst Marion Roussel, Paris, France
关键词
D O I
10.1095/biolreprod61.4.1070
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Matrix metalloproteinases (MMPs) are zinc-requiring enzymes that can degrade components of the extracellular matrix and that are implicated in tissue remodeling. Their role in the onset of menstruation in vivo has been proven; however, the expression and functions of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in vascular structures are poorly understood. We determined by immunocytochemistry, using characterized monoclonal antibodies, the distribution of MMPs and of their inhibitors TIMP-1 and TIMP-2 in the endometrium during the menstrual cycle. MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 had differing distributions and patterns of expression. In addition to the localization of MMP-9 in the epithelium and of MMP-2, MMP-3, and MMP-1 in the stromal tissue, these MMPs were detected in the vascular structures. MMP-2 (72-kDa gelatinase) and tissue inhibitors TIMP-1 and TIMP-2 were detectable in vessels throughout the cycle. In contrast, MMP-3 (stromelysin-l) was detected only in late-secretory and menstrual endometrial vessels, while MMP-9 (92-kDa gelatinase) was detected in spiral arteries during the secretory phase and in vascular structures during the midfollicular and menstrual phases. The expression of MMP-2 and MMP-9 in endometrial vessels during the proliferative and secretory periods suggests their relationship to vascular growth and angiogenesis. The pronounced expression of MMP-3 (stromelysin-l) in the vessels situated in the superficial endometrial layer during menses suggests that this metalloproteinase initiates damage in the vascular wall during menstrual breakdown. The finding of an intense expression of TIMP-1 and TIMP-2 in the vessels delineating necrotic from non-necrotic areas during menses also suggests that they could limit tissue damage, allowing regeneration of the endometrium after menses. These data indicate that, in addition to expression in epithelial cells and stromal tissue, MMPs are expressed in endometrial vascular cells in a cycle-specific pattern, consistent with regulation by steroid hormones and with specific roles in the vascular remodeling processes occurring in the endometrium during the cycle.
引用
收藏
页码:1070 / 1082
页数:13
相关论文
共 57 条
[1]   METALLOPROTEINASE AND TIMP EXPRESSION BY THE HUMAN BREAST-CARCINOMA CELL-LINE 8701-BC [J].
ALESSANDRO, R ;
MINAFRA, S ;
PUCCIMINAFRA, I ;
ONISTO, M ;
GARBISA, S ;
MELCHIORI, A ;
TETLOW, L ;
WOOLLEY, DE .
INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (02) :250-255
[2]  
Allaire E, 1997, DEV CARDIOVASC MED, P305
[3]   Paracrine action of vascular endothelial growth factor in the human endometrium: Production and target sites, and hormonal regulation [J].
Bausero P. ;
Cavaillé F. ;
Méduri G. ;
Freitas S. ;
Perrot-Applanat M. .
Angiogenesis, 1998, 2 (2) :167-182
[4]   MATRIX METALLOPROTEINASES - A REVIEW [J].
BIRKEDALHANSEN, H ;
MOORE, WGI ;
BODDEN, MK ;
WINDSOR, LJ ;
BIRKEDALHANSEN, B ;
DECARLO, A ;
ENGLER, JA .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 1993, 4 (02) :197-250
[5]   TRANSFORMING GROWTH-FACTOR-BETA MEDIATES THE PROGESTERONE SUPPRESSION OF AN EPITHELIAL METALLOPROTEINASE BY ADJACENT STROMA IN THE HUMAN ENDOMETRIUM [J].
BRUNER, KL ;
RODGERS, WH ;
GOLD, LI ;
KORC, M ;
HARGROVE, JT ;
MATRISIAN, LM ;
OSTEEN, KG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7362-7366
[6]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[7]  
DELAGE G, 1995, HUM REPROD, V10, P2483
[8]  
DOCHERTY AJP, 1990, ANN RHEUM DIS, V19, P169
[9]   FURTHER-STUDIES ON ACTIVATION OF PROCOLLAGENASE, LATENT PRECURSOR OF BONE COLLAGENASE - EFFECTS OF LYSOSOMAL CATHEPSIN-B, PLASMIN AND KALLIKREIN, AND SPONTANEOUS ACTIVATION [J].
EECKHOUT, Y ;
VAES, G .
BIOCHEMICAL JOURNAL, 1977, 166 (01) :21-31
[10]  
EECKHOUT Y, 1990, CONTRACEPTION MECHAN, P431