Mitogenic signaling mediated by oxidants in retinol treated sertoli cells

被引：40

作者：

Dal-Pizzol, F

Klamt, F

Dalmolin, RJS

Bernard, EA

Moreira, JCF

机构：

[1] Univ Fed Rio Grande Sul, ICBS, Dept Bioquim, Lab Estresse Oxidativo, BR-90035003 Porto Alegre, RS, Brazil

[2] Univ Extremo Sul Catarinense, Dept Med, Criciuma, Brazil

来源：

FREE RADICAL RESEARCH | 2001年 / 35卷 / 06期

关键词：

retinol; vitamin A; oxidative stress; TBARS; proliferation; focus;

D O I：

10.1080/10715760100301251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer. Recently the causal mechanism has begun to be clarified. We report here that retinol-induced oxidative stress is accompanied by cellular proliferation. Retinol (7 muM) significantly induced thiobarbituric acid reactive species (TBARS) formation, which was inhibited by trolox, superoxide dismutase, N-acetylcysteine and ethanol. This was accompanied by an increase in DNA synthesis and focus formation in cultured rat Sertoli cells. Antioxidants and ethanol inhibited retinol-induced DNA synthesis. Our findings suggest that retinol-induced oxidative stress was associated with cellular proliferation complementing our understanding of the significance of retinol supplementation in neoplastic transformation.

引用

页码：749 / 755

页数：7

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