DNA-templated cooperative formation of the luminous lanthanide complex and its analytical application to gene detection

被引：23

作者：

Kitamura, Y

Ihara, T

Tsujimura, Y

Tazaki, M

Jyo, A

机构：

[1] Kumamoto Univ, Dept Appl Chem & Biochem, Kumamoto 8608555, Japan

[2] Sojo Univ, Fac Engn, Kumamoto 8600082, Japan

来源：

CHEMISTRY LETTERS | 2005年 / 34卷 / 12期

关键词：

D O I：

10.1246/cl.2005.1606

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ethylenediamine tetraacetic acid (EDTA) and 1,10-phenanthrorine (phen) were covalently attached to the 5' and 3' ends of two different oligonucleotides (ODNs), respectively. The sequences of the ODN conjugates were designed to contiguously hybridize on the target DNA with their auxiliary units facing each other, providing a microenvironment to accommodate the lanthanide ions. The characteristic emissions of the Tb3+ and Eu3+ ions were clearly observed in the presence of the full-matched target DNA. On the other hand, the emissions from the mixed solutions with the mutant containing one base displacement were marginal.

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页码：1606 / 1607

页数：2

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