Priming and activation of mouse macrophages by trehalose 6,6′-dicorynomycolate vesicles from Corynebacterium glutamicum

被引：8

作者：

Chami, M

Andréau, K

Lemassu, A

Petit, JF

Houssin, C

Puech, V

Bayan, N

Chaby, R

Daffé, M

机构：

[1] Univ Paris 11, CNRS, Inst Biophys & Biochim Mol & Cellulaire, Lab Endotoxines, F-91405 Orsay, France

[2] CNRS, Inst Biophys & Biochim Mol & Cellulaire, Lab Biomembranes, F-91405 Orsay, France

[3] Univ Toulouse 3, CNRS, Inst Pharmacol & Biol Struct, F-31062 Toulouse, France

[4] CNRS, Inst Biophys & Biochim Mol & Cellulaire, Lab Monoxyde Azote Inflammat & Immunite, F-91405 Orsay, France

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2002年 / 32卷 / 02期

关键词：

Corynebacterium; trehalose dimycolate; lipopolysaccharide; macrophage; tumor necrosis factors; nitric oxide;

D O I：

10.1111/j.1574-695X.2002.tb00546.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Vesicles consisting of pure trehalose dicorynomycolate (TDCM), the corynebacterial analog of the most studied mycobacterial glycolipid 'cord factor', were isolated from Corynebacterium glutamicum cells by mild detergent treatment; these induced in vivo a macrophage priming similar to that obtained with mycobacterial-derived trehalose dimycolate. In vitro, both TDCM and bacterial lipopolysaccharide (LPS) induced in macrophages the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), endotoxin tolerance, and were primed for an enhanced secondary NO response to LPS. Interferon-gamma pretreatment did not influence the LPS-induced TNF-alpha response, but considerably increased the TDCM-induced response. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：141 / 147

页数：7

共 32 条

[1]

ADAMS DO, 1982, FED PROC, V41, P2212

[2] Involvement of the membrane form of tumour necrosis factor-alpha in lipopolysaccharide-induced priming of mouse peritoneal macrophages for enhanced nitric oxide response to lipopolysaccharide [J].