Albumin-CdTe nanoparticle bioconjugates: Preparation, structure, and interunit energy transfer with antenna effect

被引:398
作者
Mamedova, NN
Kotov, NA [1 ]
Rogach, AL
Studer, J
机构
[1] Oklahoma State Univ, Dept Chem, Stillwater, OK 74078 USA
[2] Univ Hamburg, Inst Phys Chem, D-20146 Hamburg, Germany
关键词
D O I
10.1021/nl015519n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Conjugates of bovine serum albumin and CdTe nanoparticles capped with L-cysteine have been synthesized via a one-pot glutaric dialdehyde cross-linking procedure. Diads (1:1) with some amount of 2:1 albumin-nanoparticle assemblies preferably form in this reaction, as evidenced by gel electrophoresis. Circular dichroism spectroscopy demonstrates that the tertiary structure of the protein remains largely intact after the conjugation. Attachment of protein moieties result in a significant increase of CdTe emission, which is attributed to the resonance energy transfer from the tryptophan moieties of albumin to CdTe nanoparticles acting as receptors for the protein antennae.
引用
收藏
页码:281 / 286
页数:6
相关论文
共 46 条
  • [1] Perspectives on the physical chemistry of semiconductor nanocrystals
    Alivisatos, AP
    [J]. JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (31) : 13226 - 13239
  • [2] Organization of 'nanocrystal molecules' using DNA
    Alivisatos, AP
    Johnsson, KP
    Peng, XG
    Wilson, TE
    Loweth, CJ
    Bruchez, MP
    Schultz, PG
    [J]. NATURE, 1996, 382 (6592) : 609 - 611
  • [3] [Anonymous], 1982, Soviet Physics Semiconductors USSR
  • [4] Aslam M., 1998, BIOCONJUGATION PROTE
  • [5] Nanocrystalites: Building blocks for quantum dot heterostructures
    Bawendi, MG
    [J]. SOLID STATE COMMUNICATIONS, 1998, 107 (11) : 709 - 709
  • [6] Molecular and supramolecular sensitization of nanocrystalline wide band-gap semiconductors with mononuclear and polynuclear metal complexes
    Bignozzi, CA
    Argazzi, R
    Kleverlaan, CJ
    [J]. CHEMICAL SOCIETY REVIEWS, 2000, 29 (02) : 87 - 96
  • [7] Spin-dependent tunneling in self-assembled cobalt-nanocrystal superlattices
    Black, CT
    Murray, CB
    Sandstrom, RL
    Sun, SH
    [J]. SCIENCE, 2000, 290 (5494) : 1131 - 1134
  • [8] Semiconductor nanocrystals as fluorescent biological labels
    Bruchez, M
    Moronne, M
    Gin, P
    Weiss, S
    Alivisatos, AP
    [J]. SCIENCE, 1998, 281 (5385) : 2013 - 2016
  • [9] Nanocrystals and nano-optics
    Brus, LE
    Trautman, JK
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 1995, 353 (1703): : 313 - 321
  • [10] Quantum dot bioconjugates for ultrasensitive nonisotopic detection
    Chan, WCW
    Nie, SM
    [J]. SCIENCE, 1998, 281 (5385) : 2016 - 2018