Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries

被引：35

作者：

Oni, J

Pailleret, A

Isik, S

Diab, N

Radtke, I

Blöchl, A

Jackson, M

Bedioui, F

Schuhmann, W

机构：

[1] Ruhr Univ Bochum, Lehrstuhl Mol Neurobiochem, D-44780 Bochum, Germany

[2] Ecole Natl Super Chim Paris, Lab Electrochim & Chim Analyt, UMR CNRS ENSCP 7575, F-75231 Paris 05, France

[3] Cent Res Labs Ltd, Hayes, Middx, England

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 06期

关键词：

nitric oxide; electrodes array; electrocatalysis; chemically modified electrodes; HUVEC;

D O I：

10.1007/s00216-004-2512-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In a preliminary study aimed at developing strategies for the simultaneous detection of various biologically important molecules, a procedure is described that allows the electrochemical detection of nitric oxide (NO) released by a population of human umbilical vein endothelial cells (HUVEC) by using an array of electrodes comprising three individually addressable electrodes. Each electrode in the array was modified with a different NO-sensitive electrocatalyst, thereby demonstrating the possibility of modifying the individual electrodes in an array with different sensing chemistries. This study opens a doorway to the development of arrays of electrodes for the simultaneous detection of multiple analytes in a complex environment by suitably tailoring the sensitivity and selectivity of each electrode in the array to a specific analyte in the test medium.

引用

页码：1594 / 1600

页数：7

共 24 条

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