Engineering cytochrome P450s for bioremediation

被引：54

作者：

Kellner, DG ^{[1
]}

Maves, SA ^{[1
]}

Sligar, SG ^{[1
]}

机构：

[1] UNIV ILLINOIS, DEPT BIOCHEM, URBANA, IL 61801 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 03期

关键词：

D O I：

10.1016/S0958-1669(97)80003-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Building on the vast knowledge of active site structure and catalytic mechanisms of the P450 monooxygenase systems, significant efforts to utilize the rational design of engineered P450s are emerging as an approach to solve the problems of bioremediation. P450 enzymes are being designed to alter substrate specificities and catalytic efficiency in predefined ways. In addition, random mutagenesis and in vitro evolution are being considered as exciting methods for generating mutant P450s with increased bioremediation abilities. (C) Current Biology Ltd.

引用

页码：274 / 278

页数：5

共 51 条

[1] ACTIVE-SITE ANALYSIS OF FERRIC P450 ENZYMES - HYDROGEN-BONDING EFFECTS ON THE CIRCULAR-DICHROISM SPECTRA [J].