Comparison of two isolates of Encephalitozoon hellem and E-intestinalis (Microspora) by pulsed field gel electrophoresis

被引：13

作者：

Biderre, C

Canning, EU

Méténier, G

Vivarès, CP ^{[1
]}

机构：

[1] Univ Clermont Ferrand, CNRS, UPESA 6023, Lab Protistol Mol & Cellulaire Parasites Opportun, F-63177 Clermont Ferrand, France

[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London 5W7 2AZ, England

来源：

EUROPEAN JOURNAL OF PROTISTOLOGY | 1999年 / 35卷 / 02期

关键词：

microsporidia; Encephalitozoon; molecular karyotype; polymorphism;

D O I：

10.1016/S0932-4739(99)80037-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Contour-clamped homogeneous electric field gel electrophoresis was used to separate chromosome-sized DNA from two strains each of Encephalitozoon hellem and Encephalitozoon intestinalis isolated in vitro from AIDS patients. The two isolates ofE. hellem differed significantly in molecular karyotype(EhD with 8 bands from 207 to 281 kb, and EhC with 12 bands from 175 to 315 kb), indicating a marked genetic polymorphism. The two E. intestinalis isolates displayed the same molecular karyotype with 10 bands comprised of between 182 and 279 kb. Of microsporidia so far investigated, species of Encephalitozoon have the smallest nuclear genomes (2.3-2.9 Mb).

引用

页码：194 / 196

页数：3

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