Metal chelate affinity precipitation: a new approach to protein purification

被引：32

作者：

Kumar, A ^{[1
]}

Galaev, IY ^{[1
]}

Mattiasson, B ^{[1
]}

机构：

[1] Univ Lund, Ctr Chem & Chem Engn, Dept Biotechnol, S-22100 Lund, Sweden

来源：

BIOSEPARATION | 1998年 / 7卷 / 4-5期

关键词：

metal chelate; affinity precipitation; poly(N-isopropylacrylamide-co-vinylimidazole); thermoprecipitation; cloud-point; protein purification; protein inhibitors;

D O I：

10.1023/A:1008003227597

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Metal chelate affinity precipitation of proteins, a method combining metal-protein interaction and affinity precipitation is being discussed as a selective separation process for proteins. The technique utilizes a flexible soluble-insoluble thermo-responsive polymer with a covalently linked ligand loaded with metal ions. The affinity binding of the target protein varies with different metal ions. Copolymers of N-isopropylacrylamide with 1-vinylimidazole loaded with Cu(II) ions are designed as a potential carriers for affinity purification and proved to be successful for purification of protein inhibitors from a variety of cereals.

引用

页码：185 / 194

页数：10

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