Sequence-specific assignments of methyl groups in high-molecular weight proteins

被引：45

作者：

Yang, DW

Zheng, Y

Liu, DJ

Wyss, DF

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[3] Schering Plough Res Inst, Kenilworth, NJ 07033 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 12期

关键词：

D O I：

10.1021/ja039102q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new 3D multiple-quantum (H)CCmHm-TOCSY experiment is proposed to assign methyl resonances in high-molecular weight proteins, on the basis of spectral patterns and prior backbone assignments. The favorable relaxation properties of the multiple-quantum coherences and the slow decays of in-phase methyl 13C magnetizations optimize performance of the proposed experiment for application to large proteins. The experiment has been demonstrated on an acyl carrier protein synthase (trimer, 42 kDa, overall correlation time of 26 ns) at 25 °C, and 63 out of 67 nonmethionine methyl groups have been assigned. Copyright © 2004 American Chemical Society.

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页码：3710 / 3711

页数：2

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