Carboxymethyl poly(L-histidine) as a new pH-sensitive polypeptide to enhance polyplex gene delivery

被引：48

作者：

Asayama, Shoichiro ^{[1
]}

Sudo, Miyuki ^{[1
]}

Nagaoka, Shoji ^{[1
]}

Kawakami, Hiroyoshi ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Appl Chem, Tokyo 1920397, Japan

来源：

MOLECULAR PHARMACEUTICS | 2008年 / 5卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

carboxymethyl poly(L-histidine); pH-sensitive polypeptide; polyplex; gene delivery;

D O I：

10.1021/mp800094b

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Carboxymethyl poly(L-histidine) (CM-PLH) as a new pH-sensitive polypeptide has enhanced polyplex gene delivery. Agarose gel retardation assay and zeta potential measurement proved that the anionic CM-PLH at physiological pH coated the PEI/DNA binary complexes. The resulting CM-PLH/PEI/DNA ternary complexes showed the gene expression value 300 times higher than that of the PEI/DNA binary complexes. These results suggest that the synergistic effect of the pH-sensitive imidazole groups at endosomal pH and the anionic carboxymethyl groups at physiological pH in the CM-PLH enhanced polyplex gene delivery.

引用

页码：898 / 901

页数：4

共 21 条

[1] pH-dependent dissociation of carbohydrate ligand polycations from DNA ternary complexes [J].

Asayama, S ;

Sekine, T ;

Kawakami, H ;

Nagaoka, S .

CHEMISTRY LETTERS, 2006, 35 (01) :100-101

[2] Design of aminated poly(1-vinylimidazole) for a new pH-Sensitive polycation to enhance cell-specific gene delivery [J].

Asayama, Shoichiro ;

Sekine, Takashi ;

Kawakami, Hiroyoshi ;

Nagaoka, Shoji .

BIOCONJUGATE CHEMISTRY, 2007, 18 (05) :1662-1667

[3] Carboxymethyl poly(L-histidine) as a new pH-sensitive polypeptide at endosomal/lysosomal pH [J].

Asayama, Shoichiro ;

Kato, Hiroyuki ;

Kawakami, Hiroyoshi ;

Nagaoka, Shoji .

POLYMERS FOR ADVANCED TECHNOLOGIES, 2007, 18 (04) :329-333

[4] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[5] Effect of size and serum proteins on transfection efficiency of poly((2-dimethylamino)ethyl methacrylate)-plasmid nanoparticles [J].

Cherng, JY ;

vandeWetering, P ;

Talsma, H ;

Crommelin, DJA ;

Hennink, WE .

PHARMACEUTICAL RESEARCH, 1996, 13 (07) :1038-1042

[6] Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery [J].

Dash, PR ;

Read, ML ;

Barrett, LB ;

Wolfert, M ;

Seymour, LW .

GENE THERAPY, 1999, 6 (04) :643-650

[7] POLYAMIDOAMINE CASCADE POLYMERS MEDIATE EFFICIENT TRANSFECTION OF CELLS IN CULTURE [J].

HAENSLER, J ;

SZOKA, FC .

BIOCONJUGATE CHEMISTRY, 1993, 4 (05) :372-379

[8] Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation [J].

Ito, Tomoko ;

Iida-Tanaka, Naoko ;

Niidome, Takuro ;

Kawano, Takahito ;

Kubo, Koji ;

Yoshikawa, Kenichi ;

Sato, Toshinori ;

Yang, Zhihong ;

Koyama, Yoshiyuki .

JOURNAL OF CONTROLLED RELEASE, 2006, 112 (03) :382-388

[9] EFFICIENT TRANSFORMATION OF MAMMALIAN-CELLS USING DNA INTERPOLYELECTROLYTE COMPLEXES WITH CARBON-CHAIN POLYCATIONS [J].

KABANOV, AV ;

ASTAFIEVA, IV ;

MAKSIMOVA, IV ;

LUKANIDIN, EM ;

GEORGIEV, GP ;

KABANOV, VA .

BIOCONJUGATE CHEMISTRY, 1993, 4 (06) :448-454

[10] Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells [J].

Matsui, H ;

Johnson, LG ;

Randell, SH ;

Boucher, RC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (02) :1117-1126

← 1 2 3 →