In vivo survival and osteogenic differentiation of allogeneic rat bone marrow mesenchymal stem cells (MSCs)

被引：60

作者：

Kotobuki, Noriko ^{[1
]}

Katsube, Yoshihiro ^{[1
]}

Katou, Youichi ^{[1
]}

Tadokoro, Mika ^{[1
]}

Hirose, Motohiro ^{[1
]}

Ohgushi, Hajime ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Inst Cell Engn, Amagasaki, Hyogo 6610974, Japan

来源：

CELL TRANSPLANTATION | 2008年 / 17卷 / 06期

关键词：

mesenchymal stem cells (MSCs); osteoblasts; bioengineering; implants; bone mineralization;

D O I：

10.3727/096368908786092793

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Marrow mesenchymal stein cells (MSCs) are multipotent progenitor cells and reported to be immunoprivileged as well as immunosuppressive. Hence, MSCs might be ideal candidates for allogeneic transplantation to induce regeneration of damaged tissues/organs. To confirm the differentiation capability of allogeneic MSCs in vivo is important for the acceleration of regenerative medicine. Consequently, we have established an in vivo rat model using subcutaneous implantation of a hydroxyapatite (HA) ceramic/MSCs composite. Osteogenic differentiation was used as an indicator of differentiation. When syngeneic MSCs were implanted, MSCs showed osteogenic differentiation as evidenced by new bone formation as well as high alkaline phosphatase (ALP) activity. When allogeneic MSCs were implanted, none of the allografts survived or showed osteogenic differentiation. However, when the recipient rats were treated with FK506 immunosuppressant, allogeneic MSCs showed osteogenic differentiation. Although this finding might not be adequate for the acceleration of regenerative medicine, these results did confirm that MSCs are not intrinsically immunoprivileged but that under appropriate immunosuppressant treatment, allogeneic MSCs can survive and show differentiation capability in vivo.

引用

页码：705 / 712

页数：8

共 26 条

[1] Human mesenchymal stem cells modulate allogeneic immune cell responses [J].

Aggarwal, S ;

Pittenger, MF .

BLOOD, 2005, 105 (04) :1815-1822

[2] Osteogenic phenotype expression of allogeneic rat marrow cells in porous hydroxyapatite ceramics [J].

Akahane, M ;

Ohgushi, H ;

Yoshikawa, T ;

Sempuku, T ;

Tamai, S ;

Tabata, S ;

Dohi, Y .

JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (04) :561-568

[3]

Akahane Manabu, 2002, J Orthop Sci, V7, P677, DOI 10.1007/s007760200120

[4] Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect [J].

Arinzeh, TL ;

Peter, SJ ;

Archambault, MP ;

van den Bos, C ;

Gordon, S ;

Kraus, K ;

Smith, A ;

Kadiyala, S .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2003, 85A (10) :1927-1935

[5] Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo [J].

Bartholomew, A ;

Sturgeon, C ;

Siatskas, M ;

Ferrer, K ;

McIntosh, K ;

Patil, S ;

Hardy, W ;

Devine, S ;

Ucker, D ;

Deans, R ;

Moseley, A ;

Hoffman, R .

EXPERIMENTAL HEMATOLOGY, 2002, 30 (01) :42-48

[6] Allogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice [J].

Eliopoulos, N ;

Stagg, J ;

Lejeune, L ;

Pommey, S ;

Galipeau, J .

BLOOD, 2005, 106 (13) :4057-4065

[7] STROMAL CELLS RESPONSIBLE FOR TRANSFERRING MICROENVIRONMENT OF HEMATOPOIETIC TISSUES - CLONING INVITRO AND RETRANSPLANTATION INVIVO [J].

FRIEDENSTEIN, AJ ;

CHAILAKHYAN, RK ;

LATSINIK, NV ;

PANASYUK, AF ;

KEILISSB.IV .

TRANSPLANTATION, 1974, 17 (04) :331-340

[8] THE MAJOR HISTOCOMPATIBILITY COMPLEX OF THE RAT [J].

GILL, TJ ;

KUNZ, HW ;

MISRA, DN ;

HASSETT, ALC .

TRANSPLANTATION, 1987, 43 (06) :773-785

[9] Pluripotency of mesenchymal stem cells derived from adult marrow [J].

Jiang, Yuehua ;

Jahagirdar, Balkrishna N. ;

Reinhardt, R. Lee ;

Schwartz, Robert E. ;

Keene, C. Dirk ;

Ortiz-Gonzalez, Xilma R. ;

Reyes, Morayma ;

Lenvik, Todd ;

Lund, Troy ;

Blackstad, Mark ;

Du, Jingbo ;

Aldrich, Sara ;

Lisberg, Aaron ;

Low, Walter C. ;

Lergaespada, David A. ;

Verfaillie, Catherine M. .

Nature, 2002, 418 (6893) :41-49

[10] BIOCOMPATIBILITY OF APATITE CERAMICS IN MANDIBLES [J].

KATO, K ;

AOKI, H ;

TABATA, T ;

OGISO, M .

BIOMATERIALS MEDICAL DEVICES AND ARTIFICIAL ORGANS, 1979, 7 (02) :291-297

← 1 2 3 →