Structural investigations of cross-linked hyaluronan

被引:23
作者
Gamini, A
Paoletti, S
Toffanin, R
Micali, F
Michielin, L
Bevilacqua, C
机构
[1] Univ Trieste, Dept Biochem Biophys & Macromol Chem, I-34127 Trieste, Italy
[2] Fidia SpA, Padua, Italy
关键词
cross-linked hyaluronan; SAXS; 1D-NMR microscopy; SEM; synchrotron radiation;
D O I
10.1016/S0142-9612(01)00231-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Structural properties of several cross-linked hyaluronan derivatives, obtained by scanning electron microscopy, monodimensional NMR microscopy and small angle X-ray scattering of synchrotron radiation, are presented and compared with those observed for non-modified hyaluronic acid, used as a reference material. The experimental results, obtained in different media, showed a consistent picture of the synthesized matrices. In particular. the presence of zones of denser polymeric material observed by electron microscopy resulted in a higher transversal relaxation rate of the bulk water protons as well as in a decrease of the diffusion coefficient obtained by NMR microscopy. Moreover, the presence of polymer junction zones gave rise to the appearance of a well-defined correlation peak in the pattern of intensity of the scattered X-radiation. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1161 / 1167
页数:7
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