Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function

被引:51
作者
Varma, D [1 ]
Dujardin, DL [1 ]
Stehman, SA [1 ]
Vallee, RB [1 ]
机构
[1] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10032 USA
关键词
D O I
10.1083/jcb.200510120
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Zeste white 10 (ZW10) is a mitotic checkpoint protein and the anchor for cytoplasmic dynein at mitotic kinetochores, though it is expressed throughout the cell cycle. We find that ZW10 localizes to pericentriolar membranous structures during interphase and cosediments with Golgi membranes. Dominant-negative ZW10, anti-ZW10 antibody, and ZW10 RNA interference (RNAi) caused Golgi dispersal. ZW10 RNAi also dispersed endosomes and lysosomes. Live imaging of Golgi, endosomal, and lysosomal markers after reduced ZW10 expression showed a specific decrease in the frequency of minus end-directed movements. Golgi membrane-associated dynein was markedly decreased, suggesting a role for ZW10 in dynein cargo binding during interphase. We also find ZW10 enriched at the leading edge of migrating fibroblasts, suggesting that ZW10 serves as a general regulator of dynein function throughout the cell cycle.
引用
收藏
页码:655 / 662
页数:8
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