Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission

被引：28

作者：

Nagafuchi, S

Katsuta, H

Kogawa, K

Akashi, T

Kondo, S

Sakai, Y

Tsukiyama, T

Kitamura, D

Niho, Y

Watanabe, T

机构：

[1] Kyushu Univ, Med Inst Bioregulat, Sch Hlth Sci, Dept Med Technol,Higashi Ku, Fukuoka 8128582, Japan

[2] Kyushu Univ, Med Inst Bioregulat, Dept Internal Med 1, Fukuoka 8128582, Japan

[3] Kyushu Univ, Dept Mol Immunol, Fukuoka 8128582, Japan

[4] Kyushu Univ, Med Inst Bioregulat, Dept Mol & Cellular Biol, Fukuoka 8128582, Japan

[5] Sci Univ Tokyo, Res Inst Biol Sci, Div Mol Biol, Noda, Chiba 278, Japan

来源：

FEBS LETTERS | 1999年 / 455卷 / 1-2期

关键词：

embryonic stem cell; non-obese diabetic mouse; germ line transmission; diabetes; insulitis;

D O I：

10.1016/S0014-5793(99)00801-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype, After injecting the NODES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele, When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)(g7) haplotype. Thus, an NOD-ES cell line which cao differentiate into lymphocytes with potential for germ line transmission was successfully established. (C) 1999 Federation of European Biochemical Societies.

引用

页码：101 / 104

页数：4

共 30 条

[1] Direct evidence for the contribution of B cells to the progression of insulitis and the development of diabetes in non-obese diabetic mice [J].