Performance Characteristics of the Platelia Aspergillus Enzyme Immunoassay for Detection of Aspergillus Galactomannan Antigen in Bronchoalveolar Lavage Fluid

被引：75

作者：

Husain, S. ^{[1
]}

Clancy, C. J. ^{[1
]}

Nguyen, M. H. ^{[1
]}

Swartzentruber, S.

Leather, H. ^{[2
]}

LeMonte, A. M. ^{[3
]}

Durkin, M. M. ^{[3
]}

Knox, K. S. ^{[4
]}

Hage, C. A. ^{[5
]}

Bentsen, C. ^{[6
]}

Singh, N. ^{[1
]}

Wingard, J. R. ^{[2
]}

Wheat, L. J. ^{[3
]}

机构：

[1] Univ Pittsburgh, Pittsburgh, PA USA

[2] Univ Florida, Coll Med, Gainesville, FL USA

[3] MiraVista Diagnost, Indianapolis, IN 46241 USA

[4] Univ Arizona, So Arizona VA Hlth Care Syst, Tucson, AZ USA

[5] Indiana Univ, Sch Med, Roudebush VA Med Ctr, Indianapolis, IN USA

[6] Biorad Labs, Redmond, WA USA

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2008年 / 15卷 / 12期

关键词：

D O I：

10.1128/CVI.00226-08

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have evaluated the Platelia Aspergillus enzyme immunoassay for detection of galactomannan in bronchoalveolar lavage (BAL) specimens in solid organ transplant patients with aspergillosis. The precision and reproducibility in serum or BAL to which galactomannan was added were similar. Sensitivity was 81.8% in patients with aspergillosis, and specificity was 95.8% in lung transplant patients who underwent BAL for surveillance for infection or rejection. Among transplant controls, positive results were more common in patients (i) who underwent diagnostic BAL performed for evaluation of symptoms or chest computed tomographic abnormalities, (ii) who had undergone lung transplantation, or (iii) who were colonized with Aspergillus. Galactomannan testing in BAL is useful for diagnosis of aspergillosis in transplant patients. The significance of positive results in patients without confirmed aspergillosis requires further evaluation.

引用

页码：1760 / 1763

页数：4

共 12 条

[1] Galactomannan detection in computerized tomography-based broncho-alveolar lavage fluid and serum in haematological patients at risk for invasive pulmonary aspergillosis
Becker, MJ
Lugtenburg, EJ
Cornelissen, JJ
van der Schee, C
Hoogsteden, HC
de Marie, S
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 2003, 121 (03) : 448 - 457
[2] Bronchoalveolar lavage galactomannan in diagnosis of invasive pulmonary aspergillosis among solid-organ transplant recipients
Clancy, Cornelius J.
Jaber, Reia A.
Leather, Helen L.
Wingard, John R.
Staley, Benjamin
Wheat, L. Joseph
Cline, Christina L.
Rand, Kenneth H.
Schain, Denise
Baz, Maher
Nguyen, M. Hong
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) : 1759 - 1765
[3] Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group
De Pauw, Ben
Walsh, Thomas J.
Donnelly, J. Peter
Stevens, David A.
Edwards, John E.
Calandra, Thierry
Pappas, Peter G.
Maertens, Johan
Lortholary, Olivier
Kauffman, Carol A.
Denning, David W.
Patterson, Thomas F.
Maschmeyer, Georg
Bille, Jacques
Dismukes, William E.
Herbrecht, Raoul
Hope, William W.
Kibbler, Christopher C.
Kullberg, Bart Jan
Marr, Kieren A.
Munoz, Patricia
Odds, Frank C.
Perfect, John R.
Restrepo, Angela
Ruhnke, Markus
Segal, Brahm H.
Sobel, Jack D.
Sorrell, Tania C.
Viscoli, Claudio
Wingard, John R.
Zaoutis, Theoklis
Bennett, John E.
[J]. CLINICAL INFECTIOUS DISEASES, 2008, 46 (12) : 1813 - 1821
[4] Plasmalyte as a cause of false-positive results for Aspergillus galactomannan in bronchoalveolar lavage fluid
Hage, Chadi A.
Reynolds, John M.
Durkin, Michelle
Wheat, L. Joseph
Knox, Kenneth S.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) : 676 - 677
[5] American society of transplantation recommendations for screening, monitoring and reporting of infectious complications in immunosuppression trials in recipients of organ transplantation
Humar, A
Michaels, M
[J]. AMERICAN JOURNAL OF TRANSPLANTATION, 2006, 6 (02) : 262 - 274
[6] Aspergillus galactomannan antigen in the bronchoalveolar lavage fluid for the diagnosis of invasive aspergillosis in lung transplant recipients
Husain, Shahid
Paterson, David L.
Studer, Sean M.
Crespo, Maria
Pilewski, Joseph
Durkin, Michelle
Wheat, Joseph L.
Johnson, Bruce
McLaughlin, Lisa
Bentsen, Christopher
McCuny, Kenneth R.
Singh, Nina
[J]. TRANSPLANTATION, 2007, 83 (10) : 1330 - 1336
[7] Advances in the serological diagnosis of invasive Aspergillus infections in patients with haematological disorders
Maertens, Johan
Theunissen, Koen
Lodewyck, Tom
Lagrou, Katrien
Van Eldere, Johan
[J]. MYCOSES, 2007, 50 : 2 - 17
[8] Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay
Marr, KA
Laverdiere, M
Gugel, A
Leisenring, W
[J]. CLINICAL INFECTIOUS DISEASES, 2005, 40 (12) : 1762 - 1769
[9] Galactomannan in bronchoalveolar lavage fluid - A tool for diagnosing aspergillosis in intensive care unit patients
Meersseman, Wouter
Lagrou, Katrien
Maertens, Johan
Wilmer, Alexander
Hermans, Greet
Vanderschueren, Steven
Spriet, Isabel
Verbeken, Eric
Van Wijngaerden, Eric
[J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2008, 177 (01) : 27 - 34
[10] Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
Musher, B
Fredricks, D
Leisenring, W
Balajee, SA
Smith, C
Marr, KA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (12) : 5517 - 5522

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