Cement-related particles interact with proinflammatory IL-8 chemokine from human primary oropharyngeal mucosa cells and human epithelial lung cancer cell line A549

被引:9
作者
Bauer, Mario [1 ]
Graebsch, Carolin [1 ]
Gminski, Richard [2 ]
Ollmann, Ariane I. H. [2 ]
Borm, Paul [3 ]
Dietz, Andreas [4 ]
Herbarth, Olf [5 ]
Wichmann, Gunnar [4 ]
机构
[1] UFZ Helmholtz Ctr Environm Res, Dept Environm Immunol, D-04318 Leipzig, Germany
[2] Univ Med Ctr Freiburg, Dept Environm Hlth Sci, D-79106 Freiburg, Germany
[3] Ctr Expertise Life Sci, Hgsk Zuyd, NL-6400 AN Heerlen, Netherlands
[4] Univ Leipzig, Dept Otorhinolaryngol, Fac Med, D-04103 Leipzig, Germany
[5] Univ Leipzig, Fac Med Environm Med & Hyg, D-04103 Leipzig, Germany
关键词
cement dust; A549; primary epithelial cells; human; interleukin; 8; OBSTRUCTIVE PULMONARY-DISEASE; DIESEL EXHAUST PARTICLES; RESPIRATORY SYMPTOMS; VENTILATORY FUNCTION; CYTOKINE; EXPOSURE; WORKERS; EXPRESSION; SECRETION; RELEASE;
D O I
10.1002/tox.20643
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Epidemiological studies have shown that respirable exposure to emitted cement particulate matter is associated with adverse health risk for human. The underlying mechanisms, however, are poorly understood. To examine the effect of cement, nine blinded cement-related particulates (<10 mu m) were assessed with regard to their induction of the proinflammatory cytokines IL-6 and IL-8 in human primary epithelial cells (pEC) from oropharyngeal mucosa as well as from nonsmall-cell lung carcinoma (non-SCLC) cells A549. It was demonstrated that the cement specimens did not act cytotoxic as assessed by the lactate dehydrogenase (LDH) assay. The basal and IL-1 beta-induced IL-8 expression was suppressed, in contrast to an unchanged IL-6. At the transcript level the basal and induced IL-6 and IL-8 gene expression was not influenced by cement dust. To discover the mechanism by which cement influenced the IL-8 expression the following experiments were performed. Submerse exposure experiments have shown that the release of IL-8 was suppressed by cement dust. Furthermore, the incubation of IL-8 with cement-related specimens under cell-free condition led to a loss of immunoreactive IL-8. An immunological masking of IL-8 by free soluble components of respiratory epithelial cells was excluded. Thus, the decrease of IL-8 protein content after cement exposure seems to be a result of the adsorption of IL-8 protein to cement particles and the inhibition of IL-8 release. In conclusion, due to absent cytotoxic and inflammatory effects of cement-related specimens in both human pEC and A549 cell models it remains open how cement exposure may lead to the respiratory adverse effects in humans. (C) 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.
引用
收藏
页码:297 / 306
页数:10
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