Use of SSR markers to determine the anther-derived homozygous lines in coconut

被引：27

作者：

Perera, P. I. P. ^{[1
,6
]}

Perera, L. ^{[2
]}

Hocher, V. ^{[3
]}

Verdeil, J. -L. ^{[4
]}

Yakandawala, D. M. D. ^{[5
,6
]}

Weerakoon, L. K. ^{[1
]}

机构：

[1] Coconut Res Inst, Tissue Culture Div, Lunuwila 61150, Sri Lanka

[2] Coconut Res Inst, Genet & Plant Breeding Div, Lunuwila 61150, Sri Lanka

[3] Inst Res & Dev, BEPC, UMR 1098, F-34394 Montpellier 1, France

[4] CIRAD, F-34398 Montpellier 5, France

[5] Univ Peradeniya, Dept Bot, Fac Sci, Peradeniya, Sri Lanka

[6] Univ Peradeniya, Postgrad Inst Sci, Peradeniya, Sri Lanka

来源：

PLANT CELL REPORTS | 2008年 / 27卷 / 11期

关键词：

Coconut; Androgenesis; Dihaploid; Flow cytometry; SSR marker;

D O I：

10.1007/s00299-008-0592-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.

引用

页码：1697 / 1703

页数：7

共 22 条

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