Triterpenes from Poria cocos suppress growth and invasiveness of pancreatic cancer cells through the downregulation of MMP-7

被引：92

作者：

Cheng, Shujie ^{[1
,2
]}

Eliaz, Isaac ^{[4
]}

Lin, Junfang ^{[1
]}

Sliva, Daniel ^{[2
,3
]}

机构：

[1] South China Agr Univ, Coll Food Sci, Dept Bioengn, Guangzhou 510640, Guangdong, Peoples R China

[2] Indiana Univ Hlth, Methodist Res Inst, Canc Res Lab, Indianapolis, IN 46202 USA

[3] Indiana Univ Sch Med, Dept Med, Indianapolis, IN USA

[4] Amitabha Med Clin & Healing Ctr, Sebastopol, CA USA

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2013年 / 42卷 / 06期

关键词：

triterpenes; Poria cocos; pancreatic cancer; pachymic acid; MMP-7; DUCTAL ADENOCARCINOMA; PACHYMIC ACID; MATRIX METALLOPROTEINASE-7; A549; CELLS; INHIBITION; EXPRESSION; APOPTOSIS; PROGRESSION; INDUCTION; PROGNOSIS;

D O I：

10.3892/ijo.2013.1902

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Poria cocos is a medicinal mushroom that is widely used in traditional Asian medicine. Here, we show that a characterized mixture of triterpenes extracted from P. cocos (PTE) and three purified triterpenes: pachymic acid (PA), dehydropachymic acid (DPA) and polyporenic acid C (PPAC) suppress the proliferation of the human pancreatic cancer cell lines Panc-1, MiaPaca-2, AsPc-1 and BxPc-3. Moreover, the most effective compound, PA, only slightly affects the proliferation of HPDE-6 normal pancreatic duct epithelial cells. The anti-proliferative effects of PTE on BxPc-3 cells are mediated by the cell cycle arrest at G0/G1 phase. DNA microarray analysis demonstrated that PTE significantly downregulates the expression of KRAS and matrix metalloproteinase-7 (MMP-7) in BxPc-3 cells. In addition, PTE and PA suppress the invasive behavior of BxPc-3 cells. The inhibition of invasiveness by PTE and PA was associated with the reduction of MMP-7 at the protein level and the role of MMP-7 further confirmed by the gene silencing of MMP-7 which also suppressed the invasiveness of BxPc-3 cells. In conclusion, triterpenes from P. cocos demonstrate anticancer and anti-invasive effects on human pancreatic cancer cells and can be considered as new therapeutic agents in the treatment of pancreatic cancer.

引用

页码：1869 / 1874

页数：6

共 32 条

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