Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis

被引：28

作者：

Bullido, R ^{[1
]}

Alonso, F ^{[1
]}

delMoral, MG ^{[1
]}

Ezquerra, A ^{[1
]}

Alvarez, B ^{[1
]}

Ortuno, E ^{[1
]}

Dominguez, J ^{[1
]}

机构：

[1] INIA, CTR INVEST SANIDAD ANIM, VALDEOLMOS 28130, MADRID, SPAIN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1996年 / 195卷 / 1-2期

关键词：

monoclonal antibody; granulocyte; monocyte; macrophage; CD11b; Mac-1; integrin; (Pig);

D O I：

10.1016/0022-1759(96)00095-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterization of a new mAb, named 2F4/11, specific for porcine myelomonocytic cells is described. This mAb immunoprecipitates a non-covalently linked heterodimer of 155 000/95 000, which is expressed by granulocytes, monocytes and tissue macrophages but not by lymphocytes, erythrocytes or platelets. Immunoblot analysis localizes the 2F4/11 epitope on the largest subunit of the heterodimer. Mab 2F4/11 is able to block phagocytosis of complement-opsonized zymosan particles by PMN granulocytes and alveolar macrophages, as well as adherence to plastic surfaces of PMA-activated PMN. Together, these results suggest that mAb 2F4/11 recognizes the CD11b or a chain of the porcine complement type 3 receptor (CR3).

引用

页码：125 / 134

页数：10

共 42 条

[1] ADHESIVE RECEPTOR MAC-1 COORDINATES THE ACTIVATION OF FACTOR-X ON STIMULATED CELLS OF MONOCYTIC AND MYELOID DIFFERENTIATION - AN ALTERNATIVE INITIATION OF THE COAGULATION PROTEASE CASCADE
ALTIERI, DC
MORRISSEY, JH
EDGINGTON, TS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) : 7462 - 7466
[2] ANDERSON DC, 1986, J IMMUNOL, V137, P15
[3] RELATIVE CONTRIBUTION OF THE LEUKOCYTE MOLECULES MO1, LFA-1, AND P150,95 (LEUM5) IN ADHESION OF GRANULOCYTES AND MONOCYTES TO VASCULAR ENDOTHELIUM IS TISSUE-SPECIFIC AND STIMULUS-SPECIFIC
ARNAOUT, MA
LANIER, LL
FALLER, DV
[J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1988, 137 (02) : 305 - 309
[4] INHIBITION OF PHAGOCYTOSIS OF COMPLEMENT-C3-COATED OR IMMUNOGLOBULIN-G-COATED PARTICLES AND OF C3BI BINDING BY MONOCLONAL-ANTIBODIES TO A MONOCYTE-GRANULOCYTE MEMBRANE GLYCOPROTEIN (MOL)
ARNAOUT, MA
TODD, RF
DANA, N
MELAMED, J
SCHLOSSMAN, SF
COLTEN, HR
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1983, 72 (01) : 171 - 179
[5] ANTI-MAC-1 SELECTIVELY INHIBITS THE MOUSE AND HUMAN TYPE 3 COMPLEMENT RECEPTOR
BELLER, DI
SPRINGER, TA
SCHREIBER, RD
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1982, 156 (04) : 1000 - 1009
[6] MEMBRANE-PROTEINS INVOLVED IN PHAGOCYTE ADHERENCE TO ENDOTHELIUM
CARLOS, TM
HARLAN, JM
[J]. IMMUNOLOGICAL REVIEWS, 1990, 114 : 5 - 28
[7] PRODUCTION AND TITRATION OF AFRICAN SWINE FEVER VIRUS IN PORCINE ALVEOLAR MACROPHAGES
CARRASCOSA, AL
SANTAREN, JF
VINUELA, E
[J]. JOURNAL OF VIROLOGICAL METHODS, 1982, 3 (06) : 303 - 310
[8] DAMOISEAUX J, 1991, THESIS FREE U AMSTER
[9] DANA N, 1986, J IMMUNOL, V137, P3259
[10] DANILENKO DM, 1995, J IMMUNOL, V155, P35

← 1 2 3 4 5 →