A ColE1-compatible expression vector for the production of His-tagged fusion proteins

被引：3

作者：

Barriault, D ^{[1
]}

Sylvestre, M ^{[1
]}

机构：

[1] Univ Quebec, Inst Natl Rech Sci Sante, Pointe Claire, PQ H9R 1G6, Canada

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1999年 / 75卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

biphenyl dioxygenase; pQE31; pGP1-2;

D O I：

10.1023/A:1002070630330

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmid pDB31 is a ColE1-compatible expression vector based on the p15A origin of replication. It is designed to express His-tagged fusion proteins in cells co-hosting a compatible expression vector. It was constructed by assembling the operator/promoter region plus the 6xHis and the multiple cloning site of pQE31 (QIAGEN) with the p15A origin of replication plus Kan(R) of pGP1-2. The plasmid was found to be stable in Escherichia coli strains BL21 and DH11S. It was used to produce and purify the ferredoxin reductase component of Comamonas testosteroni B-356 biphenyl dioxygenase inside a clone hosting the remaining dioxygenase genes on a compatible plasmid.

引用

页码：293 / 297

页数：5

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