Evaluation of the determinants of flow - Mediated radial artery vasodilatation in humans

The relative importance of the early peak response during hyperaemia and of the duration of the hyperaemic phase (t1/2: blood now velocity half time and AUCt1/2: area under the curve of flow velocity at t1/2) in the magnitude of the flow-dependent vasodilatation of the radial artery was determined in humans. Radial artery diameter was measured continuously in 18 healthy volunteers using an echo-tracking system coupled to a Doppler device for the measurement of the radial blood flow. In 9 subjects, arterial parameters were measured at baseline and during 3 hyperaemic tests performed after 2, 5 or 10 minutes of ischaemia. Reproducibility of the measured parameters was studied in 9 other subjects. Radial artery diameter, AUCt1/2 and t1/2 increased proportionally with the duration of ischaemia. In contrast, the peak flow response was already maximal after 5 minutes of ischaemia. The regression analysis showed that the best fit model after stepwise analysis only included t1/2 (r=0.85, p<0.001). There was no correlation between the peak flow values and the duration of hyperaemia (r=0.29, p=0.14). These results demonstrate that conduit arteries postischaemic flow-dependent vasodilatation in humans is both determined by the peak value and by the duration of the hyperaemic phase and suggest that these two components must be consirered when comparing this index of NO release between different groups of subjects.