Effect of dental materials on gluconeogenesis in rat kidney tubules

被引:17
作者
Reichl, FX
Durner, J
Mückter, H
Elsenhans, B
Forth, W
Kunzelmann, KH
Hickel, R
Spahl, W
Hume, WR
Moes, GW
机构
[1] Univ Munich, Walther Straub Inst Pharmakol & Toxikol, D-80336 Munich, Germany
[2] Univ Munich, Dept Operat Restorat Dent, D-80336 Munich, Germany
[3] Univ Munich, Inst Organ Chem, D-81377 Munich, Germany
[4] Univ Calif Los Angeles, Dent Res Inst, Los Angeles, CA 90095 USA
[5] TNO, Prins Maurits Lab, NL-2280 AA Rijswijk, Netherlands
关键词
triethyleneglycoldimethacrylate (TEGDMA); 2-hydroxyethylmethacrylate (HEMA); dental material; gluconeogenesis; kidney cells;
D O I
10.1007/s002040050677
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA) as well as mercuric chloride (HgCl2) and methylmercury chloride (MeHgCl) on gluconeogenesis was investigated in isolated rat kidney tubules. From starved rats kidney tubules were prepared and isolated by digestion with collagenase. Every 10 min up to 60 min l-ml samples were drawn from the cell suspension for quantitating the glucose content. Glucose formation in controls was 3.3 +/- 0.2 nmol/mg.per min (mean +/- SEM, n = 21). Relative rates of glucose formation were obtained by expressing individual rates as a percentage of the corresponding control. X-Y concentration curves (effective concentration, EC) of the substances were calculated by fitting a four-parametric sigmoid function to the relative rates of glucose formation at various test concentrations. At the end of the incubation period cell viability was assessed by trypan blue exclusion. Cell viability decreased within the 60 min interval from 90 to approx. 80% (controls), < 25 (HEMA), < 20 (TEGDMA), < 10 (MeHgCl), and <10% (HgCl2). Values of 50% effective concentration (EC50) were calculated from fitted curves. EC50 values were (mmol; mean +/- SEM; n = 4). HEMA, 17.7 +/- 2.9; TEGDMA, 1.8 +/- 0.2; MeHgCl, 0.018 +/- 0.0005; and HgCl2, 0.0016 +/- 0.0005. The toxic effect of HgCl2 was similar to 1000 or 10 000 higher than that of the dental composite components TEGDMA or HEMA, respectively.
引用
收藏
页码:381 / 386
页数:6
相关论文
共 48 条
[1]  
BEISENHERZ G, 1953, Z NATURFORSCH B, V8, P555
[2]   Hepatotoxic effects of SH-reagents in human and rat hepatocyte cultures and in situ perfused rat livers [J].
Boot, JH .
CELL STRUCTURE AND FUNCTION, 1996, 21 (04) :221-229
[3]  
Bowen R.L, 1965, U.S. Patent, Patent No. [3194784, US3194784A 13]
[4]  
Bowen R. L., 1962, U. S. Pat., Patent No. [3,066,112, 3066112]
[5]  
BOWEN RL, 1965, Patent No. 3194783
[6]  
Denny MF, 1996, NEUROTOXICOLOGY, V17, P47
[7]   CURRENT TRENDS IN DENTAL COMPOSITES [J].
FERRACANE, JL .
CRITICAL REVIEWS IN ORAL BIOLOGY AND MEDICINE, 1995, 6 (04) :302-318
[8]  
FORST HT, 1985, ARZNEIMITTEL-FORSCH, V35-1, P563
[9]   H-1 AND C-13 NMR-STUDIES OF THE INTERACTION OF EUGENOL, PHENOL, AND TRIETHYLENEGLYCOL DIMETHACRYLATE WITH PHOSPHOLIPID LIPOSOMES AS A MODEL SYSTEM FOR ODONTOBLAST MEMBRANES [J].
FUJISAWA, S ;
KADOMA, Y ;
KOMODA, Y .
JOURNAL OF DENTAL RESEARCH, 1988, 67 (11) :1438-1441
[10]  
GERZINA T, 1991, Journal of Dental Research, V70, P424