Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D

被引：73

作者：

Ella, KM

Dolan, JW

Qi, C

Meier, KE

机构：

[1] MED UNIV S CAROLINA, DEPT CELL & MOLEC PHARMACOL, CHARLESTON, SC 29425 USA

[2] MED UNIV S CAROLINA, DEPT MICROBIOL IMMUNOL, CHARLESTON, SC 29425 USA

来源：

BIOCHEMICAL JOURNAL | 1996年 / 314卷

关键词：

SMOOTH-MUSCLE CELLS; PHOSPHATIDIC-ACID; DEPENDENT ACTIVATION; PHORBOL ESTERS; GROWTH-FACTOR; PROTEIN; PHOSPHATIDYLCHOLINE; YEAST; ENZYME;

D O I：

10.1042/bj3140015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.

引用

页码：15 / 19

页数：5

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