Intracellular degradation of C-peptides in molluscan neurons producing insulin-related hormones

被引：14

作者：

deWith, ND ^{[1
]}

Li, KW ^{[1
]}

Jimenez, CR ^{[1
]}

Vonk, N ^{[1
]}

Dreisewerd, K ^{[1
]}

Hillenkamp, F ^{[1
]}

Karas, M ^{[1
]}

Geraerts, WPM ^{[1
]}

机构：

[1] UNIV MUNSTER, INST MED PHYS & BIOPHYS, D-48149 MUNSTER, GERMANY

来源：

PEPTIDES | 1997年 / 18卷 / 06期

关键词：

mollusca; Lymnaea stagnalis; insulin-related peptides; C-peptides; matrix assisted laser desorption mass; spectrometry (MALDI-MS); electrospray mass spectrometry; amino acid sequence analysis;

D O I：

10.1016/S0196-9781(97)00020-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single Light Green Cells (LGC) of Lymnaea stagnalis, expressing four genes encoding insulin-related peptides (MIPs) and C-peptides, and sections from the median lip nerve (MLN) were subjected to MALDI-MS. Mass spectra of LGCs and MLNs were almost identical. Masses corresponding to those of the MIPs and some C-alpha-peptides could be distinguished. ProMIP III C-alpha-peptide and C-beta-peptides were not found. The spectra showed additional masses matching those of carboxyterminally truncated C-alpha-peptides. Peptides with similar masses were isolated from MLN extracts by HPLC, using electrospray-MS screening. Amino acid sequence analysis revealed intact proMIP I, II and V C-alpha-peptides and I, II C-alpha-peptide 1-24, 1-22 and 1-15. (C) 1997 Elsevier Science Inc.

引用

页码：765 / 770

页数：6

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