The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment

被引:121
作者
Marra, P [1 ]
Maffucci, T
Daniele, T
Di Tullio, G
Ikehara, Y
Chan, EKL
Luini, A
Beznoussenko, G
Mironov, A
De Matteis, MA
机构
[1] Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Santa Maria Imbaro, Chieti, Italy
[2] Fukuoka Univ, Sch Med, Dept Biochem, Fukuoka 8140180, Japan
[3] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
关键词
D O I
10.1038/ncb1201-1101
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Integrating the pleomorphic membranes of the intermediate compartment (IC) into the array of Golgi cisternae is a crucial step in membrane transport, but it is poorly understood. To gain insight into this step, we investigated the dynamics by which cis-Golgi matrix proteins such as GM130 and GRASP65 associate with, and incorporate, incoming IC elements. We found that GM130 and GRASP65 cycle via membranous tubules between the Golgi complex and a constellation of mobile structures that we call late IC stations. These stations are intermediate between the IC and the cis-Golgi in terms of composition, and they receive cargo from earlier IC elements and deliver it to the Golgi complex. Late IC elements are transient in nature and sensitive to fixatives; they are seen in only a fraction of fixed cells, whereas they are always visible in living cells. Finally, late IC stations undergo homotypic fusion and establish tubular connections between themselves and the Golgi. Overall, these features indicate that late IC stations mediate the transition between IC elements and the cis-Golgi face.
引用
收藏
页码:1101 / 1113
页数:13
相关论文
共 50 条
  • [1] Rab1 recruitment of p115 into a cis-SNARE complex: Programming budding COPII vesicles for fusion
    Allan, BB
    Moyer, BD
    Balch, WE
    [J]. SCIENCE, 2000, 289 (5478) : 444 - 448
  • [2] The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic
    Alvarez, C
    Garcia-Mata, R
    Hauri, HP
    Sztul, E
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (04) : 2693 - 2700
  • [3] ER to Golgi transport: Requirement for p115 at a pre-Golgi VTC stage
    Alvarez, C
    Fujita, H
    Hubbard, A
    Sztul, E
    [J]. JOURNAL OF CELL BIOLOGY, 1999, 147 (06) : 1205 - 1221
  • [4] Membrane dynamics at the endoplasmic reticulum Golgi interface
    Bannykh, SI
    Balch, WE
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 138 (01) : 1 - 4
  • [5] Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae
    Barr, FA
    Nakamura, N
    Warren, G
    [J]. EMBO JOURNAL, 1998, 17 (12) : 3258 - 3268
  • [6] Barros L.C., 1997, P 7 IFSA WORLD C, V2, P3
  • [7] TRANSCYTOSIS-ASSOCIATED PROTEIN (TAP)/P115 IS A GENERAL FUSION FACTOR REQUIRED FOR BINDING OF VESICLES TO ACCEPTOR MEMBRANES
    BARROSO, M
    NELSON, DS
    SZTUL, E
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (02) : 527 - 531
  • [8] Blum R, 2000, J CELL SCI, V113, P3151
  • [9] SNARE membrane trafficking dynamics in vivo
    Chao, DS
    Hay, JC
    Winnick, S
    Prekeris, R
    Klumperman, J
    Scheller, RH
    [J]. JOURNAL OF CELL BIOLOGY, 1999, 144 (05) : 869 - 881
  • [10] De Matteis MA, 2000, J CELL SCI, V113, P2331