Calibration and application of an X-ray image intensifier/charge-coupled device detector for monochromatic macromolecular crystallography

被引：59

作者：

Hammersley, AP ^{[1
]}

Brown, K ^{[1
]}

Burmeister, W ^{[1
]}

Claustre, L ^{[1
]}

Gonzalez, A ^{[1
]}

McSweeney, S ^{[1
]}

Mitchell, E ^{[1
]}

Moy, JP ^{[1
]}

Svensson, SO ^{[1
]}

Thompson, AW ^{[1
]}

机构：

[1] EMBL OUTSTN, F-38042 GRENOBLE, FRANCE

来源：

JOURNAL OF SYNCHROTRON RADIATION | 1997年 / 4卷

关键词：

detector calibration; data collection; detector distortions; X-ray detectors; charge-coupled device detectors; macromolecular crystallography;

D O I：

10.1107/S0909049596015087

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Charge-coupled device (CCD)-based X-ray detectors allow data to be collected much more quickly (similar to 10 times) than with current on-line imaging-plate systems. At the ESRF, X-ray image intensifier/CCD detector systems have been developed. These have great potential as fast read-out detectors for macromolecular and other forms of crystallography. They are relatively large sensitive X-ray detectors but have two inherent weaknesses: convex detection surfaces leading to spatial distortion and non-uniformity of intensity response, and susceptibility to small changes in magnetic fields. A large improvement has been made to the accuracy obtained by non-uniformity of response calibration and correction, using fluorescence from doped lithium borate glasses. Monochromatic macromolecular crystallography demonstration experiments with external user groups have shown that high-quality results may be obtained under real experimental conditions.

引用

页码：67 / 77

页数：11

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