Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

被引：13

作者：

Grieshaber, Dorothee ^{[1
]}

de Lange, Victoria ^{[1
]}

Hirt, Thomas ^{[2
]}

Lu, Zhihua ^{[2
]}

Voeroes, Janos ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Lab Biosensors & Bioelect, Inst Biomed Engn, CH-8092 Zurich, Switzerland

[2] BioCure Inc, Norcross, GA 30071 USA

来源：

SENSORS | 2008年 / 8卷 / 12期

关键词：

High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance;

D O I：

10.3390/s8127894

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.

引用

页码：7894 / 7903

页数：10

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