Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis

被引：61

作者：

Iguchi, N

Tanaka, H

Fujii, T

Tamura, K

Kaneko, Y

Nojima, H

Nishimune, Y

机构：

[1] Osaka Univ, Microbial Dis Res Inst, Dept Sci Lab Anim Experimentat, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Microbial Dis Res Inst, Dept Mol Genet, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 1999年 / 456卷 / 02期

关键词：

mouse; testis; tektin; cDNA library; sperm;

D O I：

10.1016/S0014-5793(99)00967-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules, We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the moose testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity. (C) 1999 Federation of European Biochemical Societies.

引用

页码：315 / 321

页数：7

共 28 条

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