Isolation and structural elucidation of a novel homogenous polysaccharide from Tricholoma matsutake

被引：34

作者：

Cheng, Hua ^{[1
]}

Jia, Yan ^{[1
]}

Wang, Ling ^{[1
]}

Liu, Xiaoying ^{[2
]}

Liu, Guangrong ^{[2
]}

Li, Li ^{[1
]}

He, Congfen ^{[1
]}

机构：

[1] Beijing Technol & Business Univ, Sch Sci, Beijing Key Lab Plants Resource Res & Dev, Beijing 100048, Peoples R China

[2] Infinitus Int China Co Ltd, Guangzhou 510665, Guangdong, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2016年 / 30卷 / 01期

关键词：

polysaccharide; Tricholoma matsutake; GC-MS; 2D-NMR; structure elucidation;

D O I：

10.1080/14786419.2015.1034711

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from Tricholoma matsutake by water extraction and alcohol precipitation. From this crude polysaccharide, a homogeneous polysaccharide, TMP-5II, was successfully purified by Sephacryl S-300 column chromatography. The average molecular weight (M-w) of TMP-5II was 15.76kDa. Monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues: d-glucose, d-galactose, d-mannose and d-fucose. Attenuated total reflectance infrared spectroscopy revealed characteristics typical of carbohydrate polymers and a peak typical of a -type glycosidic bond. TMP-5II was selected for structural characterisation by nuclear magnetic resonance (NMR) analysis. According to H-1 NMR, C-13 NMR and two-dimensional-NMR analysis, TMP-5II contains two kinds of linkages, and , at a ratio of 4:1. Preliminary results indicated that the polysaccharide had (1-4)-beta-pyran glucose as the main chain, and a branched chain in the O-6 location with fucose (1-2) mannose (1-3)-alpha-pyran galactose.

引用

页码：58 / 64

页数：7

共 19 条

[1]

[Anonymous], THESIS

[2]

Polysaccharides from Caulerpa racemosa:: Purification and structural features [J].