Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens

被引：273

作者：

Cheng, Nan ^{[1
,3
,4
]}

Song, Yang ^{[1
]}

Zeinhom, Mohamed M. A. ^{[1
,5
]}

Chang, Yu-Chung ^{[1
]}

Sheng, Lina ^{[2
]}

Li, Haolin ^{[4
]}

Du, Dan ^{[1
]}

Li, Lei ^{[1
]}

Zhu, Mei-Jun ^{[2
]}

Luo, Yunbo ^{[3
,4
]}

Xu, Wentao ^{[3
,4
]}

Lin, Yuehe ^{[1
]}

机构：

[1] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA

[2] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA

[3] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China

[4] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R China

[5] Beni Suef Univ, Fac Vet Med, Food Hyg Dept, Bani Suwayf 62512, Egypt

来源：

ACS APPLIED MATERIALS & INTERFACES | 2017年 / 9卷 / 46期

关键词：

lateral flow immunoassay; Pt-Pd nanoparticles; milk; Salmonella Enteritidis; E. coli O157:H7; smartphone; ESCHERICHIA-COLI O157H7; LATERAL FLOW ASSAYS; COLORIMETRIC DETECTION; FOODBORNE PATHOGENS; GRAPHENE OXIDE; NANOPARTICLES; STRIP; MILK; GLUCOSE; SYSTEM;

D O I：

10.1021/acsami.7b12734

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, mesoporous core-shell palladium@platinum (Pd@Pt) nanoparticles were synthesized and then applied as signal amplifier in a dual lateral flow immunoassay (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and Escherichia coli O157:H7. After optimization, the limit of detections were calculated to be similar to 20 cfu/mL for S. Enteritidis and similar to 34 cfu/mL for E. coli O157:H7, respectively. The greatly improved sensitivity was contributed by the peroxidase-like catalytic activity of the Pd@Pt nanoparticles for signal enhancement and the parallel design of dual detection for eliminating the cross-interference. The estimated recoveries of the dual LFIA range from 91.44 to 117.00%, which indicated that the developed method is capable of detecting live bacteria in food samples. This approach provides an attractive platform for S. Enteritidis and E. coli 0157:H7 detection using a smartphone-based device as the sole piece of equipment, indicating great promise for foodborne pathogen analysis or infield food safety tracking.

引用

页码：40671 / 40680

页数：10

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