Self-Assembled BolA-like Amphiphilic Peptides as Viral-Mimetic Gene Vectors for Cancer Cell Targeted Gene Delivery

被引：38

作者：

Chen, Jing-Xiao ^{[1
,2
,3
]}

Xu, Xiao-Ding ^{[1
,2
]}

Yang, Shuo ^{[1
,2
]}

Yang, Juan ^{[1
,2
]}

Zhuo, Ren-Xi ^{[1
,2
]}

Zhang, Xian-Zheng ^{[1
,2
]}

机构：

[1] Wuhan Univ, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[3] Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China

来源：

MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 01期

基金：

国家杰出青年科学基金;

关键词：

BolA-like peptides; gene delivery; tumor targeting; ARGININE-RICH PEPTIDES; DRUG-DELIVERY; THERAPY; NANOPARTICLES; STRATEGIES; BARRIERS; SYSTEMS; DESIGN; INTERNALIZATION; MECHANISMS;

D O I：

10.1002/mabi.201200283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, two types of BolA-like amphiphilic peptides with dual ligands comprising a tumor-targeting moiety of RGD sequence and a cell-penetrating moiety of R8 sequence are designed and synthesized as gene vectors. The BolA-structural peptide carriers can self-assemble into spherical nanoparticles with a hydrophilic core and shell, which are similar to the viral capsid and can bind plasmid DNA in an aqueous medium to form viral-mimetic complexes. It is found that the BolA-like dual ligands system exhibits significantly enhanced gene expression in both HeLa and 293T cell lines, as compared with poly(ethylenimine) PEI. These BolA-like amphiphilic peptides are promising in clinical trials of gene therapy.

引用

页码：84 / 92

页数：9

共 49 条

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