Rapid Separation of Gangliosides Using Strong Anion Exchanger Cartridges

被引：7

作者：

Kato, Tomohisa ^{[2
,3
]}

Kasuya, Maria Carmelita Z. ^{[1
]}

Hatanaka, Kenichi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[2] Kaneka Corp, Kita Ku, Osaka 5308288, Japan

[3] Japan Chem Innovat Inst, Chiyoda Ku, Tokyo 1010051, Japan

来源：

JOURNAL OF OLEO SCIENCE | 2008年 / 57卷 / 07期

关键词：

ganglioside; separation; solid phase extraction;

D O I：

10.5650/jos.57.397

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method utilizing strong anion exchanger cartridges (InertSep SAX) was developed to separate gangliosides. Total lipids extracted from rat brain is able to be rapidly separated into neutral and acidic lipids rapidly. Neutral lipids were passed through the SAX cartridge while acidic lipids adsorbed onto the cartridge and were eluted by chloroform/methanol/4.0 M aqueous ammonium acetate (5:10:1, by volume). Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) were separated individually according to their characteristics by elution with increasing concentration of ammnonium acetate (0 - 4.0 M). The gangliosides yield of this procedure was higher than 95%.

引用

页码：397 / 400

页数：4

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