Molecular weight determination of lignosulfonates by size-exclusion chromatography and multi-angle laser light scattering

被引：123

作者：

Fredheim, GE

Braaten, SM

Christensen, BE

机构：

[1] Norwegian Univ Sci & Technol, Dept Biotechnol, Norwegian Biopolymer Lab, NOBIPOL, NO-7491 Trondheim, Norway

[2] Ostfold Coll, NO-1705 Sarpsborg, Norway

[3] Borregaard Ind Ltd, NO-1701 Sarpsborg, Norway

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 942卷 / 1-2期

关键词：

molecular mass determination; multi-angle laser light scattering; lignosulfonates;

D O I：

10.1016/S0021-9673(01)01377-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A lignosulfonate sample was fractionated according to the solubility in ethanol-water. The fractions were analysed by aqueous size exclusion chromatography (SEC) combined with in-line multi-angle laser light scattering (MALLS), and by static MALLS. Satisfactory SEC results were obtained with aqueous phosphate buffer containing DMSO and SDS. The refractive index increment (dn/dc) varied from 0.186 to 0.205 ml/g, depending on M-w and the degree of sulfonation. The second viral coefficient (A(2)) was 7 x 10(-3) ml mol/g(2). The weight-average molecular weight (M-w) of the fractions varied from 4600 up to 398 000 g/mol, and the polydispersity (M-w/M-n) varied between 1.3 and 3.5. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：191 / 199

页数：9

共 23 条

[1] SOME PROPERTIES OF PAUCIDISPERSE GYMNOSPERM LIGNIN SULFONATES OF DIFFERENT MOLECULAR-WEIGHTS [J].

BUCHHOLZ, RF ;

NEAL, JA ;

MCCARTHY, JL .

JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY, 1992, 12 (04) :447-469

[2] DETERMINATION OF MOLECULAR-WEIGHT AND ITS DISTRIBUTION OF LIGNOSULFONATE FROM CA-BISULFITE SPENT LIQUOR [J].

BUDIN, D ;

SUSA, L .

HOLZFORSCHUNG, 1982, 36 (01) :17-22

[3] Aqueous gel permeation chromatographic methods for technical lignins [J].

Chen, FG ;

Li, J .

JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY, 2000, 20 (03) :265-276

[4] Macromolecular characterisation of three barley β-glucan standards by size-exclusion chromatography combined with light scattering and viscometry:: an inter-laboratory study [J].

Christensen, BE ;

Ulset, AS ;

Beer, MU ;

Knuckles, BE ;

Williams, DL ;

Fishman, ML ;

Chau, HK ;

Wood, PJ .

CARBOHYDRATE POLYMERS, 2001, 45 (01) :11-22

[5]

FELICETTA VF, 1956, CAN J CHEM, V78, P1899

[6]

FORSS K, 1967, PAPERI JA PUU, V8, P525

[7]

Forss KG, 1976, APPL POLYM S, V3, P1185

[8]

FORSS KG, 1969, PAPPER TRA, V1, P93

[9]

Hjerde T, 1999, BIOPOLYMERS, V49, P71, DOI 10.1002/(SICI)1097-0282(199901)49:1<71::AID-BIP7>3.3.CO

[10]

2-8

← 1 2 3 →