Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse

被引：140

作者：

Choi, Yu Suk

Cha, Sang Myun

Lee, Young Yeol

Kwon, Seok Woon

Park, Chan Jeoung

Kim, MiJung ^{[1
]}

机构：

[1] Univ Ulsan, Asan Inst Life Sci, Seoul 138736, South Korea

[2] Sein Aesthet Plast Surg Clin, Seoul, South Korea

[3] Han Yang Univ, Sch Med, Dept Hematol, Seoul, South Korea

[4] Univ Ulsan, Coll Med, Dept Lab Med, Seoul 138736, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 345卷 / 02期

关键词：

tissue engineering; adipose tissue derived adult stem cell; PLGA; injectable scaffold;

D O I：

10.1016/j.bbrc.2006.04.128

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We evaluated the use of a combination of adipose tissue derived adult stem cells (ADSCs) obtained from liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for adipose tissue engineering. Adipogenesis was examined in nude mice injected subcutaneously with ADSCs (group I), PLGA spheres (group II), or ADSCs attached PLGA spheres (group IIIu) cultured in adipogenic medium for 7 days. After 4 and 8 weeks, newly formed adipose tissue was observed in groups II and III but not in group I. Oil red O staining of newly formed tissue showed that there was Substantially more tissue regeneration and adipogenic differentiation in group III than ill group II. RT-PCR confirmed that, after 8 weeks, the PLGA-attached ADSCs had fully differentiated into adipocytes. This Study provides significant evidence that ADSCs and PLGA spheres can be used in a clinical setting to generate adipose tissue as a noninvasive soft tissue filler. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：631 / 637

页数：7

共 28 条

[1]

Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: Implications in soft tissue augmentation and reconstruction [J].