The Lipid Portion of Activated Platelet-Rich Plasma Significantly Contributes to Its Wound Healing Properties

被引:30
作者
Hoeferlin, Lauren Alexis [1 ,2 ]
Huynh, Quoc K. [2 ]
Mietla, Jennifer A. [2 ]
Sell, Scott A. [1 ,4 ]
Tucker, Jason [1 ]
Chalfant, Charles Edward [1 ,2 ,5 ,6 ]
Wijesinghe, Dayanjan Shanaka [1 ,3 ,6 ]
机构
[1] Hunter Holmes McGuire Vet Adm Med Ctr, Richmond, VA USA
[2] Virginia Commonwealth Univ, Dept Biochem & Mol Biol, Richmond, VA 23298 USA
[3] Virginia Commonwealth Univ, Dept Surg, 1101 East Marshall St,POB 980614, Richmond, VA 23298 USA
[4] St Louis Univ, Dept Biomed Engn, Pk Coll Engn & Technol, St Louis, MO 63103 USA
[5] Virginia Commonwealth Univ, Med Coll Virginia, Massey Canc Ctr, Richmond, VA 23298 USA
[6] Virginia Commonwealth Univ, Victoria Johnson Ctr Crit Care Res, Richmond, VA 23298 USA
基金
美国国家卫生研究院;
关键词
GROWTH-FACTORS; FIBROBLASTS; ACID; PROLIFERATION; CHEMOTAXIS; LIPOXINS; RECEPTOR; RELEASE; PATHWAY; FLUID;
D O I
10.1089/wound.2014.0589
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100227 [皮肤病学];
摘要
Objective: Platelet-rich plasma (PRP) is a popular choice for the treatment of chronic wounds. Current dogma attributes these healing properties to the peptide growth factors of PRP. However, PRP is also rich in bioactive lipids whose contribution to healing has not been characterized and warrants investigation due to the protease-rich environment of chronic wounds. Approach: The lipid fraction of PRP was tested with respect to proliferation and migration of primary adult human dermal fibroblasts (HDFa) +/- exposure to chronic wound fluid (CWF). This fraction was also characterized via LC-MS/MS for bioactive lipids. A synthetic formulation of the bioactive lipid composition was developed and tested for the ability to overcome proliferative growth arrest induced by CWF. Results: The data demonstrate the ability of the lipid fraction of PRP to significantly enhance the migration and proliferation of HDFa, and to overcome the proliferative growth arrest induced by CWF. Furthermore, the synthetic lipid formulation generated following characterization of the PRP lipidome demonstrated a similar ability to overcome proliferative arrest of HDFa in the presence of CWF. Innovation: For the first time, we demonstrate the relevance of the lipid fraction of PRP toward the biology of wound healing. These studies open the possibility of altering the lipid profile of PRP via diet or exogenous pathway manipulation to obtain a better healing outcome. Conclusion: The lipid fraction of PRP is under investigated and yet relevant component in wound healing. The current study demonstrates the relevance of this fraction in wound healing by PRP.
引用
收藏
页码:100 / 109
页数:10
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