Alanine check points in HNN and HN(C)N spectra

被引：19

作者：

Chatterjee, Amarnath ^{[1
]}

Kumar, Ashutosh ^{[1
]}

Hosur, Ramakrishna V. ^{[1
]}

机构：

[1] Tata Inst Fundamental Res, Dept Chem Sci, Bombay 400005, Maharashtra, India

来源：

JOURNAL OF MAGNETIC RESONANCE | 2006年 / 181卷 / 01期

关键词：

HNN-A; HN(C)N-A; check points; triplets of residues; band selective pulse;

D O I：

10.1016/j.jmr.2006.03.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid resonance assignment is a key requirement in structural genomics research by NMR. In this context we present here two new pulse sequences, namely, HNN-A and HN(C)N-A that have been developed by simple modification of the previously described pulse sequences, HNN and HN(C)N [S.C. Panchal, N.S. Bhavesh, R.V. Hosur, Improved 3D triple resonance experiments, HNN and HN(C)N, for H-N and N-15 sequential correlations in (C-13, N-15) labeled proteins: application to unfolded proteins, J. Biomol. NMR, 20 (2001) 135-147]. These increase the number of start/check points in HNN and/or HN(C)N spectra and hence help in pacing up resonance assignment in proteins. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：21 / 28

页数：8

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