Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development

被引:446
作者
Noh, B
Murphy, AS
Spalding, EP
机构
[1] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA
[2] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
关键词
D O I
10.1105/tpc.13.11.2441
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabidopsis possesses several genes related to the multidrug resistance (MDR) genes of animals, one of which, AtMDR1, was shown to be induced by the hormone auxin. Plants having mutations in AtMDR1 or its closest relative, AtPGP1, were isolated by a reverse genetic strategy. Auxin transport activity was greatly impaired in atmdr1 and atmdr1 atpgp1 double mutant plants. Epinastic cotyledons and reduced apical dominance were mutant phenotypes consistent with the disrupted basipetal flow of auxin. The auxin transport inhibitor 1-naphthylphthalamic acid was shown to bind tightly and specifically to AtMDR1 and AtPGP1 proteins. The results indicate that these two MDR-like genes of Arabidopsis encode 1-naphthylphthalamic acid binding proteins that are required for normal auxin distribution and auxin-mediated development.
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收藏
页码:2441 / 2454
页数:14
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