Multiresidue analysis of anabolic compounds in bovine hair by gas chromatography-tandem mass spectrometry

被引：50

作者：

Marcos, V ^{[1
]}

Perogordo, E ^{[1
]}

Espinosa, P ^{[1
]}

de Pozuelo, MM ^{[1
]}

Hooghuis, H ^{[1
]}

机构：

[1] Ctr Nacl Alimentac, Inst Salud Carlos III, Unidad Residuos Zoosanitarios, Agcy Espanola Seguridad Alimentaria, Madrid 28220, Spain

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 507卷 / 02期

关键词：

hair; anabolics; residues; GC-MS-MS; analysis;

D O I：

10.1016/j.aca.2003.11.026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and specific multiresidue method was developed to perform screening and confirmation of 15 anabolics steroids in bovine hair, namely: dienestrol, DES, hexestrol, 17alpha- and 17beta-estradiol, 17alpha- and 17beta-19-nortestosterone, 17alpha- and 17beta-boldenone, testosterone (beta) and epitestosterone (alpha), ethynylestradiol, alpha-methyltestosterone, and alpha- and beta-Zearalanol. After methanolic and solid-phase extraction, trimethylsilyl derivatization (TMS) was,performed and the derivatized extracts submitted to GC-MS-MS analysis. TMS derivatives of the studied hormones yielded specific MS-MS fragmentation with a high abundance for ions selected in the screening procedure. Validation was performed regarding qualitative parameters: decision limit (M), detection capability (CCbeta) and specificity. For confirmation, European union criteria 2002/657/EC were considered. Detection capability ranged between 1.0 and 10.0 ng g(-1) depending on the compound. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：219 / 227

页数：9

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