Changing potency by spontaneous fusion

被引:1153
作者
Ying, QL
Nichols, J
Evans, EP
Smith, AG
机构
[1] Univ Edinburgh, Ctr Genome Res, Edinburgh EH9 3JQ, Midlothian, Scotland
[2] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
基金
英国医学研究理事会; 美国国家卫生研究院; 英国生物技术与生命科学研究理事会;
关键词
D O I
10.1038/nature729
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Recent reports have suggested that mammalian stem cells residing in one tissue may have the capacity to produce differentiated cell types for other tissues and organs(1-9). Here we define a mechanism by which progenitor cells of the central nervous system can give rise to non-neural derivatives. Cells taken from mouse brain were co-cultured with pluripotent embryonic stem cells. Following selection for a transgenic marker carried only by the brain cells, undifferentiated stem cells are recovered in which the brain cell genome has undergone epigenetic reprogramming. However, these cells also carry a transgenic marker and chromosomes derived from the embryonic stem cells. Therefore the altered phenotype does not arise by direct conversion of brain to embryonic stem cell but rather through spontaneous generation of hybrid cells. The tetraploid hybrids exhibit full pluripotent character, including multilineage contribution to chimaeras. We propose that transdetermination consequent to cell fusion 10 could underlie many observations otherwise attributed to an intrinsic plasticity of tissue stem cells(9).
引用
收藏
页码:545 / 548
页数:4
相关论文
共 29 条
  • [1] AKESON EC, 1996, GENETIC VARIANTS STR, P1506
  • [2] EMBRYONIC STEM-CELLS EXPRESS NEURONAL PROPERTIES IN-VITRO
    BAIN, G
    KITCHENS, D
    YAO, M
    HUETTNER, JE
    GOTTLIEB, DI
    [J]. DEVELOPMENTAL BIOLOGY, 1995, 168 (02) : 342 - 357
  • [3] BARSKI G, 1961, J NATL CANCER I, V26, P1269
  • [4] Turning brain into blood: A hematopoietic fate adopted by adult neural stem cells in vivo
    Bjornson, CRR
    Rietze, RL
    Reynolds, BA
    Magli, MC
    Vescovi, AL
    [J]. SCIENCE, 1999, 283 (5401) : 534 - 537
  • [5] The evolving concept of a stem cell: Entity or function?
    Blau, HM
    Brazelton, TR
    Weimann, JM
    [J]. CELL, 2001, 105 (07) : 829 - 841
  • [6] From marrow to brain: Expression of neuronal phenotypes in adult mice
    Brazelton, TR
    Rossi, FMV
    Keshet, GI
    Blau, HM
    [J]. SCIENCE, 2000, 290 (5497) : 1775 - 1779
  • [7] Generalized potential of adult neural stem cells
    Clarke, DL
    Johansson, CB
    Wilbertz, J
    Veress, B
    Nilsson, E
    Karlström, H
    Lendahl, U
    Frisén, J
    [J]. SCIENCE, 2000, 288 (5471) : 1660 - 1663
  • [8] DOETSCHMAN TC, 1985, J EMBRYOL EXP MORPH, V87, P27
  • [9] Ephrussi Boris, 1972, HYBRIDIZATION SOMATI
  • [10] Muscle regeneration by bone marrow derived myogenic progenitors
    Ferrari, G
    Cusella-De Angelis, G
    Coletta, M
    Paolucci, E
    Stornaiuolo, A
    Cossu, G
    Mavilio, F
    [J]. SCIENCE, 1998, 279 (5356) : 1528 - 1530