Time-resolved electron spin resonance studies of spin-labelled lipids in membranes

被引：51

作者：

Bartucci, Rosa ^{[1
]}

Erilov, Denis A.

Guzzi, Rita

Sportelli, Luigi

Dzuba, Sergei A.

Marsh, Derek

机构：

[1] Univ Calabria, Dipartimento Fis, I-87036 Cosenza, Italy

[2] ETH, Inst Mol Biol & Biophys, Zurich, Switzerland

[3] Russian Acad Sci, Inst Chem Kinet & Combust, Novosibirsk 630090, Russia

[4] Max Planck Inst Biophys Chem, Spekt Abt, D-37077 Gottingen, Germany

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2006年 / 141卷 / 1-2期

关键词：

electron spin resonance; electron spin echo; spin labels; librational chain motions; transmembrane water penetration;

D O I：

10.1016/j.chemphyslip.2006.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Recently, developments in time-resolved spin-label electron spin resonance (ESR) spectroscopy have contributed considerably to the study of biomembranes. Two different applications of electron spin echo spectroscopy of spin-labelled phospholipids are reviewed here: (1) the use of partially relaxed echo-detected ESR spectra to study the librational lipid-chain motions in the low-temperature phases of phospholipid bilayers; (2) the use of electron spin echo envelope modulation spectroscopy to determine the penetration of water into phospholipid membranes. Results are described for phosphatidylcholine bilayer membranes, with and without equimolar cholesterol, that are obtained with phosphatidylcholine spin probes site-specifically labelled throughout the sn-2 chain. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：142 / 157

页数：16

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