Induction of glial cell line-derived neurotrophic factor receptor proteins in cerebral cortex and striatum after permanent middle cerebral artery occlusion in rats

被引：42

作者：

Kitagawa, H

Sasaki, C

Zhang, WR

Sakai, K

Shiro, Y

Warita, H

Mitsumoto, Y

Mori, T

Abe, K

机构：

[1] Okayama Univ, Sch Med, Dept Neurol, Okayama 7008558, Japan

[2] Otsuka Pharmaceut, Tokushima New Drug Res Inst, Kawauchi, Tokushima 7710192, Japan

来源：

BRAIN RESEARCH | 1999年 / 834卷 / 1-2期

关键词：

GFR alpha-1; ret; immunoreactivity; focal ischemia; middle cerebral artery occlusion;

D O I：

10.1016/S0006-8993(99)01563-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In an attempt to elucidate whether glial cell line-derived neurotrophic factor (GDNF) receptors are induced after ischemic brain injury, possible expression of immunoreactive GDNF receptor-alpha 1 (GFR alpha-1) and c-ret (RET) was examined at 3, 8, or 24 h after permanent middle cerebral artery occlusion (MCAO) in rats. Immunohistochemical study showed that both GFR alpha-1 and RET staining cells which were not detected in sham control brain, were present in the ipsilateral cortex and caudate at 3 to 8 h after permanent MCAO, and then decreased but remained to some extent at 24 h. Positive cells for both GDNF receptors were predominantly in cortical neurons of ischemic penumbral area. Western blot analysis confirmed the induction of those receptors after permanent MCAO. This rapid induction of GFR alpha-1 and RET, which correlates with the similar induction of GDNF under these conditions, may play a role in the early response to ischemic brain injury. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：190 / 195

页数：6

共 23 条

[1] Expression of the glial cell line-derived neurotrophic factor gene in rat brain after transient MCA occlusion [J].