PRELYTIC STIMULATION OF TARGET AND EFFECTOR CELLS FOLLOWING CONJUGATION AS MEASURED BY INTRACELLULAR FLUORESCEIN FLUORESCENCE POLARIZATION

被引：8

作者：

Fixler, Dror ^{[1
]}

Tirosh, Reuven ^{[1
]}

Eisenthal, Avi ^{[2
]}

Lalchuk, Shlomo ^{[3
]}

Marder, Oleg ^{[2
]}

Irlin, Yosef ^{[4
]}

Deutsch, Mordechai ^{[1
]}

机构：

[1] Bar Ilan Univ, Jerome Schottenstein Cellscan Ctr Early Detect Ca, Dept Phys, IL-52900 Ramat Gan, Israel

[2] Tel Aviv Univ, Sackler Sch Med, Sourasky Med Ctr, Inst Pathol, IL-69978 Tel Aviv, Israel

[3] Tel Aviv Univ, Beilinson Hosp, Sackler Sch Med, Petah Tiqwa, Israel

[4] Coll Judea & Samaria, Res Inst, IL-44824 Kedumim Ariel, Israel

来源：

JOURNAL OF BIOMEDICAL OPTICS | 1998年 / 3卷 / 03期

关键词：

fluorescence polarization; stimulation; effector and target cells; Cellscan;

D O I：

10.1117/1.429858

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to detect prelytic intracellular changes induced in target and effector cells following their conjugation at room temperature. Changes in the cytoplasmic matrix were measured by means of intracellular fluorescein fluorescence polarization (IFFP) using the Cellscan apparatus. Both natural killer and lymphocyte activated killer cells were used as effector cells, while K562 and Daudi cell lines were used as targets. The results show that following their conjugation, both the effector and the target cells show significant reductions ( > 10%) in IFFP values. Changes in IFFP were induced by specific interaction and only between viable cells. No evidence of fluorescein transfer from a stained cell to its nonstained counterpart was found. To the best of our knowledge, this is the first time that effector-target interaction is monitored on an individual cell basis within a population, by means of IFFP measurements. In addition, in order to explain the physical phenomena, measurements of physical parameters which might affect the IFFP, such as changes in osmolality and pH, were performed and discussed. (C) 1998 Society of Photo-Optical Instrumentation Engineers.

引用

页码：312 / 325

页数：14

共 59 条

[1] F-19-NMR STUDY OF PRIMARY HUMAN T-LYMPHOCYTE ACTIVATION - EFFECTS OF MITOGEN ON INTRACELLULAR PH [J].