Mobilization of iron from cellular ferritin by ascorbic acid in neuroblastoma SK-N-SH cells: An EPR study

被引：37

作者：

Baader, SL

Bill, E

Trautwein, AX

Bruchelt, G

Matzanke, BF

机构：

[1] UNIV LUBECK,ISOTOPENLABOR TECH NAT WISSENSCH FAK,D-23538 LUBECK,GERMANY

[2] UNIV ULM,DEPT ANAT & CELL BIOL,D-89069 ULM,GERMANY

[3] UNIV LUBECK,INST PHYS,D-23538 LUBECK,GERMANY

[4] UNIV TUBINGEN,CHILDRENS HOSP,DEPT HEMATOL & ONCOL,D-72070 TUBINGEN,GERMANY

来源：

FEBS LETTERS | 1996年 / 381卷 / 1-2期

关键词：

neuroblastoma; iron release; ferritin; ascorbic acid; electron paramagnetic resonance;

D O I：

10.1016/0014-5793(96)00098-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mobilization of iron from intracellular ferritin by ascorbic acid has been analysed in situ by electron paramagentic resonance (EPR) spectroscopy. EPR enables a distinction between ferritins and other Fe3+-binding cellular components, The ordered iron core of ferritin gives rise to a resonance signal which can be observed only at temperatures above 50 K. In the present study we clearly demonstrate that ascorbic acid is capable of mobilizing iron from ferritin in the cellular system by reduction of the ferric ion core in neuroblastoma SK-N-SH cells. This mechanism may open new ways in the therapy of this hardly curable tumor in stage IV, especially in combination with some cytostatic drugs.

引用

页码：131 / 134

页数：4

共 45 条

[1] ASCORBIC-ACID-MEDIATED IRON RELEASE FROM CELLULAR FERRITIN AND ITS RELATION TO THE FORMATION OF DNA STRAND BREAKS IN NEUROBLASTOMA-CELLS [J].