Expression of neutral invertase in sugarcane

被引：26

作者：

Bosch, S

Grof, CPL

Botha, FC

机构：

[1] Univ Stellenbosch, Inst Plant Biotechnol, ZA-7602 Matieland, South Africa

[2] CSIRO Plant Ind, Indooroopilly, Qld 4068, Australia

[3] S African Sugar Expt Stn, ZA-4300 Mt Edgecombe, South Africa

来源：

PLANT SCIENCE | 2004年 / 166卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

neutral invertase; cDNA; expression; sequence; transcript levels;

D O I：

10.1016/j.plantsci.2003.09.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conflicting reports of the distribution of sugarcane neutral invertase (SNI) activity in sugarcane, and the complete lack of genetic information have been the impetus for this investigation. Using a hetrologous sequence from Lolium temulentum a clone, which contained a 1716 bp open reading frame, was isolated from a mature culm sugarcane cDNA library. The SNI amino acid sequence had at least 53% homologous with sequences from Daucus carota and L. temulentum neutral invertases. The predicted molecular weight of the polypeptide is 64 300 Da. Southern blot analysis showed that SNI represents a low copy number gene in sugarcane. Both the SNI transcript and protein were present in all analysed tissues of the sugarcane plants and the data are consistent with transcriptional control of SNI activity. In culm tissues where sucrose content was low and hexose contents were high, SNI transcript and protein levels were higher than in tissues dedicated to sucrose storage. (C) 2003 Published by Elsevier Ireland Ltd.

引用

页码：1125 / 1133

页数：9

共 26 条

[1] SUCROSE METABOLISM IN SUGARCANE GROWN UNDER VARYING CLIMATIC CONDITIONS - SYNTHESIS AND STORAGE OF SUCROSE IN RELATION TO THE ACTIVITIES OF SUCROSE SYNTHASE, SUCROSE PHOSPHATE SYNTHASE AND INVERTASE [J].