Validation of markers linked to maximum root length in rice (Oryza sativa L.)

被引：3

作者：

Chaitra, J

Vinod, MS

Sharma, N

Hittalmani, S

Shashidhar, HE ^{[1
]}

机构：

[1] Univ Agr Sci Bangalore, Marker Assisted Select Lab, Dept Genet & Plant Breeding, GKVK, Bangalore 560065, Karnataka, India

[2] Int Rice Res Inst, Biometr & Bioinformat Unit, Los Banos, Philippines

来源：

CURRENT SCIENCE | 2006年 / 90卷 / 06期

关键词：

maximum root length; marker-assisted selection; molecular markers; polymerase chain reaction; single-marker analysis;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Root characters/traits are difficult to record and require destructive sampling of the plants. DNA-based molecular markers represent a non-destructive method for gathering information regarding the root characteristics. Two root length specific markers, BH14 and RM201 were utilized to determine the maximum root length of 81 diverse genotypes. Polymerase chain reaction analysis revealed all deep-rooted cultivars amplifying 1.57 kb and 140 bp band and shallow-rooted cultivars 1.40 kb and 158 bp band for BH14 and RM201, respectively. Single marker analysis statistically linked the markers to maximum root length, BH14 (R-2 22.24%, P = 0.0001) and RM201 (R-2 14.62%, P = 0.0004).

引用

页码：835 / 838

页数：4

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